Peer-reviewed papers

2018 - 2019
[423] Palm, G.J., Reisky, L., Böttcher, D., Müller, H., Michels, E., Walczak, M. C., Berndt, L., Weiss, M.S., Bornscheuer, U.T., Weber, G. (2019), Structure of the plastic-degrading I. sakaiensis MHETase bound to a substrate, Nat. Commun., 10, 1717, link.
[422] Ressmann, A.K., Schwendenwein, D., Leonhartsberger, S., Mihovilovic, M.D., Bornscheuer, U.T., Winkler, M., Rudroff, F. (2019), Substrate independent high-throughput assay for the quantification of aldehydes, Adv. Synth. Catal., link.
[421] Schwendenwein, D., Ressmann, A.K., Dörr, M., Höhne, M., Mihovilovic, M.D., Bornscheuer, U.T., Rudroff, F., Winkler, M., (2019), Random mutagenesis driven improvement of carboxylate reductase activity using an amino benzamidoxime-mediated high-throughput assay, Adv. Synth. Catal., link.
[420] Bornscheuer, UT. (2019), Chemie-Nobelpreis 2018 für das Design maßgeschneiderter Proteine, Chemie in unserer Zeit, accepted.
[419] Zhao, Z.X, Lan, D., Tang, X., Hollmann, F., Bornscheuer, U.T., Yang, B., Wang, Y. (2019), How to break the Janus effect of H2O2 in biocatalysis? Understanding inactivation mechanisms to generate more robust enzymes, ACS Catal., 9, 2916-2921, link.
[418] Matassa, C., Ormerod, D., Bornscheuer, U.T., Höhne, M., Satyawali, Y. (2019) Application of novel high molecular weight amine donors in chiral amine synthesis facilitates integrated downstream processing and provides in situ product recovery, Proc. Biochem., 80, 17-25link.
[417] Stamm, A., Biundo, A., Schmidt, B., Brücher, J., Lundmark, S., Olsen, P., Fogelström, L., Malmström, E., Bornscheuer, U.T., Syren, P.E. (2019), A retrobiosynthesis-based route to generate pinene-derived polyesters, ChemBioChem, link.
[416] Srinivasamurthy, V.S.T., Böttcher, D., Bornscheuer, U.T. (2019), A multi-enzyme cascade reaction for the production of 6-hydroxyhexanoic acid. Z. Naturforsch., 74, 71-76, link.
[415] Reisky, L., Srinivasamurthy, V.S.T., Badenhorst, C.P.S., Godehard, S.P., Bornscheuer, U.T. (2019), A novel high-throughput assay enables the direct identification of acyltransferases, Catalysts, 9, 64, link.
[414] Lee, D.S., Song, J.W., Voß, M., Schuiten, E., Kwon, Y.U., Bornscheuer, U., Park, J.B. (2019) Enzyme cascade reactions for the biosynthesis of long chain aliphatic amines from renewable fatty acids, Adv. Synth. Catal., 361, 1359–1367, link.
[413] Chuaboon, L., Wongnate, T., Punthong, P., Lawan, N., Kiattisewee, C., Hsu, C.-Y., Lin, C.H., Bornscheuer, U.T., Chaiyen, P. (2019), One-pot bioconversion of L-arabinose to L-ribulose in an enzymatic cascade, Angew. Chem. Int. Ed., 58, 2428-2432; Angew. Chem., 131, 2450-2454, link.
[412] Aumala, V., Mollerup, F., Jurak, E., Blume, F., Karppi, J., Koistinen, A.E., Schuiten, E., Voß, M., Bornscheuer, U., Deska, J., Master, E.R. (2019), Biocatalytic production of amino-carbohydrates through oxidoreductase and transaminase cascades, ChemSusChem, 12, 848-857, link
[411] Zorn, K., Oroz-Guinea, I., Bornscheuer, U.T. (2019) Strategies for enriching erucic acid from Crambe abyssinica oil by improved Candida antarctica lipase A variants, Proc. Biochem., 79, 65-73, link.
[410] Mattos, M.V.M., Costa I.C.R., de Souza, R.O.M.A., Bornscheuer, U.T. (2019), Biocatalytic cascade reaction for the asymmetric synthesis of L- and D-homoalanine, ChemCatChem, 11, 407-411, link.
[409] Bornscheuer, U.T., Hauer, B., Jaeger, K.E., Schwaneberg, U. (2019), Directed evolution empowered redesign of natural proteins for sustainable production of chemicals and pharmaceuticals, Angew. Chem. Int. Ed., 58, 36-40, link; Gerichtete Evolution ermöglicht das Design von maßgeschneiderten Proteinen zur nachhaltigen Produktion von Chemikalien und Pharmazeutika, Angew. Chem., 131, 36-41, link.
[408] Voss, M., Das, D., Genz, M., Kumar, A., Kulkarni, N., Kustosz, J., Kumar, P., Bornscheuer, U.T., Höhne, M. (2018), In silico based engineering approach to improve transaminases for the conversion of bulky substrates, ACS Catal., 8, 11524-11533, link.
[407] Robb, C.S, Reisky, S., Bornscheuer, U.T, Hehemann, J.H. (2018), Specificity and mechanism of carbohydrate demethylation by cytochrome P450 monooxygenases, Biochem. J., 475, 3875-3886, link.
[406] Schaaf, P., Baeyer, T., Koley, M., Schnürch, M., Bornscheuer, U., Rudroff, F., Mihovilovic, M. (2018), Biocompatible metal-assisted C-C cross-coupling combined with biocatalytic chiral reductions in a concurrent tandem cascade, Chem. Commun., 54, 12978-12981, link.
[405] Zorn, K. Oroz-Guinea, I., Brundiek, H., Dörr, M., Bornscheuer, U.T. (2018) Alteration of chain length selectivity of Candida antarctica lipase A by semi-rational design for the enrichment of erucic and gondoic fatty acids, Adv. Synth. Catal., 360, 4115-4131, link.
[404] Dawood, A.W.H., Bassut, J., de Souza, R.O.M.A., Bornscheuer, U.T. (2018) Combination of the Suzuki-Miyaura-cross coupling reaction with engineered transaminases, Chem. Eur. J., 24, 16009-16013, link.
[403] Dawood, A.W.H., Weisz, M.S., Schulz, C., Pavlidis, I.V., Iding, H., de Souza, R.O.M.A., Bornscheuer, U.T. (2018), Isopropylamine as amine donor in transaminase-catalyzed reactions: better acceptance through reaction and enzyme engineering, ChemCatChem, 10, 3943-3949, link.
[402] Morlock, L.K., Grobe, S., Balke, K., Mauersberger, S., Böttcher, D., Bornscheuer, U.T., Protein engineering of the progesterone hydroxylating P450-monooxygenase CYP17A1 alters its regioselectivity, ChemBioChem, 19, 1954-1958, link.
[401] Reisky, L., Stanetty, C., Mihovilovic, M.D, Schweder, T., Hehemann, J.H., Bornscheuer, U.T. (2018), Biochemical characterization of an ulvan lyase from the marine flavobacterium Formosa agariphila KMM 3901T, Appl. Microbiol. Biotechnol., 102, 6987-6996, link.
[400] Buß, O., Voß, M., Delavault, A., Gorenflo, P., Syldatk, C., Bornscheuer, U.T., Rudat, J. (2018), β-phenylalanine ester synthesis from stable β-keto ester substrate using engineered ω-transaminases, Molecules, 23, 1211, link.
[399] Reisky, L., Büchsenschütz, H.C., Engel, J., Song, T., Schweder, T., Hehemann, J.H., Bornscheuer, U.T. (2018), Oxidative demethylation of algal carbohydrates by cytochrome P450 monooxygenases, Nat. Chem. Biol., 14, 342-344, link.
[398] Badenhorst C.P.S., Bornscheuer, U.T. (2018) Getting momentum: from biocatalysis to advanced synthetic biology, Trends Biochem. Sci. , 43, 180-198, link.
[397] Rudroff, F., Mihovilovic, M.D., Gröger, H., Snajdrova, R., Iding, H., Bornscheuer, U.T. (2018), Opportunities and challenges for combining chemo- and biocatalysis, Nat. Catal. , 1, 12-22, link.
[396] Dawood, A.W.H., de Souza, R.O.M.A., Bornscheuer, U.T. (2018), Asymmetric synthesis of chiral halogenated amines using amine transaminases, ChemCatChem, 10, 951-955, link.
[395] Obitte, N., Zorn, K., Oroz-Guinea, I., Bornscheuer, U.T., Klein, S. (2018), Enzymatically modified shea butter and palm kernel oil as potential lipid drug delivery matrices, Eur. J. Lipid Sci. Technol., , 120, 1700332, link.
[394] Balke, K., Beier, A., Bornscheuer, U.T. (2018), Hot spots in the protein engineering of Baeyer-Villiger monooxygenases, Biotechnol. Adv., 36, 247-263, link.
[393] Morlock, L.K., Böttcher, D., Bornscheuer, U.T. (2018), Simultaneous detection of NADPH consumption and H2O2 production using the Ampliflu Red assay for screening of P450 activities and uncoupling, Appl. Microbiol. Biotechnol., 102, 985-994, link.
[392] Bornscheuer, U.T. (2018), The 4th wave of biocatalysis is approaching, Phil. Trans. R. Soc. A. , 376, 20170063, link.
[391] Bornscheuer, U.T. (2018), Enzymes in lipid modification, Ann. Rev. Food Sci. Technol., 9, 85-103, link.
[390] Bordewick, S. Beier, A, Balke, K., Bornscheuer, U.T. (2018), Baeyer-Villiger monooxygenases from Yarrowia lipolytica catalyze preferentially sulfoxidations, Enzyme Microb. Technol., 109, 31-42, link.
[389] Kohl, A., Srinivasamurthy, V., Böttcher, D., Kabisch, J., Bornscheuer, U.T. (2018), Co-expression of an alcohol dehydrogenase and a cyclohexanone monooxygenase for cascade reactions facilitates the regeneration of the NADPH cofactor, Enzyme Microb. Technol., 108, 53-58, link.
2016 - 2017
[388] Calvelage, S., Dörr, M., Höhne, M., Bornscheuer, U.T. (2017), A systematic analysis of the substrate scope of (S)- and (R)-selective amine transaminases, Adv. Synth. Catal., 359, 4235-4243, link.
[387] Costa, I.C.R., de Souza, R.O.M.A., Bornscheuer, U.T. (2017), Asymmetric synthesis of serinol-monoesters catalyzed by amine transaminases, Tetrah.: Asymmetry, 28, 1183-1187, link.
[386] Stein, N., Gumataotao, N., Hajnas, N., Wu, R., Lankathilaka, K.P., Bornscheuer, U.T., Liu, D., Fiedler, A.T., Holz, R.C., Bennett, B. (2017), Multiple states of nitrile hydratase from Rhodococcus equi TG328-2: Structural and mechanistic insights from EPR and DFT studies, Biochemistry, 56, 3068-3077, link.
[385] de Souza, R.O.M.A., Miranda, L.S.M., Bornscheuer, U.T. (2017), A retrosynthetic approach for biocatalysis in organic synthesis, Chem. Eur. J., 23, 12040-12063, link.
[384] Balke, K., Bäumgen, M., Bornscheuer, U.T. (2017) Controlling the regioselectivity of Baeyer-Villiger monooxygenases by mutation of active site residues, ChemBioChem, 8, 1627-1638, link.
[383] van den Bergh, T., Tamo, G., Nobili, A., Tao, Y., Tan, T., Bornscheuer, U.T., Kuipers, R.R.K.P., Vroling, B., de Jong, R., Subramanian, V., Schaap, P.J., Desmet, T., Nidetzky, B., Vriend, G., Joosten, H.J. (2017) CorNet: Assigning function to networks of co-evolving residues by automated literature mining, PLOS One, 12, e0176427, link.
[382] Kotapati, H.K., Robinson, J.D., Lawrence, D.R., Fortner, K.R., Stanford, C.W., Powell, D.R., Wardenga, R., Bornscheuer, U.T., Masterson, D.S. (2017), Diastereoselective hydrolysis of branched malonate diesters by porcine liver esterase: synthesis of 5-benzyl substituted Cα-methyl-β-proline, Eur. J. Org. Chem., 3009-3016, href="http://dx.doi.org/10.1002/ejoc.201700605">link.
[381] Milker, S., Fink, M.J., Oberleitner, N., Ressmann, A.K., Mihovilovic, M.D., Bornscheuer, U.T., Rudroff, F. (2017) Kinetic modeling of an enzymatic redox cascade in vivo reveals cofactor-caused bottlenecks, ChemCatChem, 9, 3420-3427, link.
[380] Schwarte, A., Genz M., Skalden, L., Nobili, A., Vickers, C., Melse, O., Kuipers, R., Joosten, H.J., Stourac, J., Bendl, J., Black, J., Baakman, C., Damborsky, J., Bornscheuer, U., Vriend, G., Venselaar, H. (2017) NewProt – a protein engineering portal, Prot. Eng. Des. Sel., 30, 441-447, link.
[379] Weiß, M.S., Pavlidis, I.V., Genz., M., Spurr, P., Hanlon, S.P., Wirz, B. Iding, H., Bornscheuer, U.T. (2017), Amine transaminase engineering for spatially bulky substrate acceptance, ChemBioChem, 18, 1022-1026, link.
[378] Scherkus, C., Schmidt, S., Bornscheuer, U., Gröger, H., Kara, S., Liese, A. (2017) Kinetic insights into ε-caprolactone synthesis: improvement of an enzymatic cascade reaction, Biotechnol. Bioeng., 114, 1215-1221, link.
[377] Wedde, S., Rommelmann, P., Scherkus, C., Schmidt, S., Bornscheuer, U.T., Liese, H., Gröger H. (2017), An alternative approach towards poly-ε-caprolactone through a chemoenzymatic synthesis: combined hydrogenation, bio-oxidations and polymerization without isolation of intermediates, Green Chem., 19, 1286-1290, link.
[376] Zühlke, M.K., Schlüter, R., Mikolasch, A., Zühlke, D., Giersberg, M., Schindler, H., Henning, A.K., Frenzl, H., Hammer, E., Lalk, M., Bornscheuer, U.T., Riedel, K., Kunze, G., Schauer, F. (2017), Biotransformation and reduction of estrogenicity of bisphenol A by the biphenyl-degrading Cupriavidus basilensis, Appl. Microbiol. Biotechnol., 101, 3743-3758, link.
[375] Böttcher, D., Zägel, P., Schmidt, M., Bornscheuer, U.T. (2017), A microtiter plate-based assay to screen for active and stereoselective hydrolytic enzymes in enzyme libraries, Meth. Mol. Biol., 1539, 197-204, link.
[374] Rudroff, F., Fink, M.J., Pydi, R., Bornscheuer, U.T., Mihovilovic, M.D. (2017), First chemo-enzymatic synthesis of the (R)-Taniguchi lactone and substrate profiles of CAMO and OTEMO, two new Baeyer-Villiger monooxygenases, Monatsh. Chem./Chem. Month., 148, 157-165, link.
[373] Knight, A.M., Nobili, A., van den Bergh, T., Genz, M., Joosten, H.J., Albrecht, D., Riedel, K., Pavlidis, I.V., Bornscheuer, U.T. (2017), Bioinformatic analysis of fold-type III PLP-dependent enzymes discovers multimeric racemases, Appl. Microbiol. Biotechnol., 101, 1499-1507, link.
[372] Joo, J.C., Khusnutdinova, A.N., Flick, R., Kim, T., Bornscheuer, U.T., Yakunin, A.F., Mahadevan, R. (2017), Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid, Chem. Sci., 8, 1406-1413, link.
[371] Peters, C., Rudroff, F., Mihovilovic, M.D., Bornscheuer, U.T. (2017), Fusion proteins of an enoate reductase and a Baeyer-Villiger monooxygenase facilitate the synthesis of chiral lactones, Biol. Chem., 398, 31-37, link.
[370] Oberleitner, N., Ressmann, A.K., Bica, K., Gärtner, P., Fraaije, M.W., Bornscheuer, U.T., Rudroff, F., Mihovilovic, M. D. (2017) From waste to value - Direct utilization of limonene from orange peel in a biocatalytic cascade reaction towards chiral carvolactone, Green Chem., 19, 367-371, link.
[369] Reimer, A., Wedde, S., Staudt, S., Schmidt, S., Höffer, D., Hummel, W., Kragl, U., Bornscheuer, U., Gröger, H. (2017), Process development through solvent engineering in the biocatalytic synthesis of the heterocyclic bulk chemical ε-caprolactone, J. Heterocycl. Chem., 54, 391-396, link.
[368] Bornscheuer, U. T. (2016), A radical change in enzyme catalysis, Nature, 540, 345-346, link.
[367] Cuetos, A., Steffen-Munsberg, F., Mangas Sanchez, J., Frese, A., Bornscheuer, U.T., Höhne, M., Grogan, G. (2016), Structural basis for phospholyase activity of a Class III transaminase homolog, ChemBioChem, 17, 2308-2311, link.
[366] Beier, A., Bordewick, S., Genz, M., Schmidt, S., van den Bergh, T., Peters, C., Joosten, H.J., Bornscheuer, U.T. (2016), Switch in cofactor specificity of a Baeyer-Villiger Monooxygenase, ChemBioChem, 17, 2312-2315, link.
[365] Scherkus, C., Schmidt, S., Bornscheuer, U., Gröger, H., Kara, S., Liese, A. (2016) A fed-batch synthetic strategy for a multi-enzymatic synthesis of poly-ε-caprolactone, ChemCatChem, 8, 3446-3452, link.
[364] Scherkus, C., Schmidt, S., Bornscheuer, U., Gröger, H., Kara, S., Liese, A. (2016) A fed-batch synthetic strategy for a multi-enzymatic synthesis of poly-ε-caprolactone, ChemCatChem, 8, 3446-3452, link.
[363] Genz, M., Okke, M., Schmidt, S., Vickers, C., Dörr, M., van den Bergh, T., Joosten, H.J., Bornscheuer, U.T. (2016), Engineering the amine transaminase from Vibrio fluvialis towards branched-chain substrates, ChemCatChem, 8, 3199-3202, link.
[362] Bauch, M., Böttcher, D., Bornscheuer, U.T., Linker, T. (2016), Enzymatic cleavage of aryl acetates, ChemCatChem, 8, 2853-2857, link.
[361] Pavlidis, I.V., Weiß, M.S., Genz., M., Spurr, P., Hanlon, S.P., Wirz, B. Iding, H., Bornscheuer, U.T. (2016), Identification of (S)-selective transaminases for the asymmetric synthesis of bulky chiral amines, Nature Chem., 8, 1076-1082, link.
[360] Song, J.W., Woo, J.M., Jung, G. Y., Bornscheuer, U.T., Park, J.B. (2016), 3'-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli, Sci. Reports,6, 29406, link.
[359] Zorn, K., Oroz-Guinea, I., Brundiek, B., Bornscheuer, U.T. (2016), Engineering and application of enzymes for lipid modification, an update, Prog. Lipid Res., 63, 153-164, link.
[358] Hinze, J., Süss, P., Bornscheuer, U.T., Wardenga, R., von Langermann, J. (2016), Recombinant pig liver esterase-catalyzed synthesis of (1S,4R)-4-hydroxy-2-cyclopentenyl acetate combined with subsequent enantioselective crystallization, Org. Proc. Res. Dev., 20, 1258-1264, link.
[357] Last, D., Krüger, G.H.E., Dörr, M. Bornscheuer, U.T. (2016), Fast, continuous and high-throughput (bio)chemical activity assay for N-acyl-L-homoserine lactone quorum quenching enzymes, Appl. Environm. Microbiol., 82, 4145-4154, link.
[356] Steffen-Munsberg, F., Matzel, P., Sowa, M., Berglund, P., Bornscheuer, U.T., Höhne, M. (2016), Bacillus anthracis ω-amino acid:pyruvate transaminase employs a different mechanism for dual substrate recognition than other amine transaminases, Appl. Microb. Biotechnol., 100, 4511-4521, link.
[355] Bornscheuer, U.T. (2016), Feeding on plastics, Science, 351, 1155-1156, link.
[354] Dörr, M., Fibinger, M.P.C., Last, D., Schmidt, S., Santos-Aberturas, J., Vickers, C., Voss, M., Bornscheuer, U.T. (2016), Fully automatized high-throughput enzyme library screening using a robotic platform, Biotechnol. Bioeng., 113, 1421-1432, link.
[353] de Souza, S. P., Junior, I. I., Miranda, L.S.M., Santiago, M.F. Lam,, F.L.Y. Dawood, A., Bornscheuer, U.T., de Souza, R.O.M.A. (2016), Cellulose as an efficient matrix for lipase and transaminase immobilization, RSC Adv., 6, 6665-6671, link.
[352] Bornscheuer, U.T. (2016), Beating the odds, Nat. Chem. Biol., 12, 54-55, link.
[351] Last, D., Müller, J., Dawood, A.W.H., Moldenhauer, E.J., Pavlidis, I.P., Bornscheuer, U.T. (2016), Highly efficient and easy protease-mediated protein purification, Appl. Microb. Biotechnol., 100, 1945–1953, link.
[350] Bornscheuer, U. T. (2016), Biocatalysis: successfully crossing boundaries, Angew. Chem. Int. Ed., 55, 4372-4373, link; Biokatalyse: ein erfolgreicher Blick über den Tellerrand, Angew. Chem., 128, 4446-4447, link.
[348] Balke, K., Schmidt, S., Genz, M., Bornscheuer, U.T. (2016), Switching the regioselectivity of a cyclohexanone monooxygenase towards (+)-trans-dihydrocarvone by rational protein design, ACS Chem. Biol., 11, 38-43, link.
[347] Choojit, S., Bornscheuer, U.T., Kittikun, A. (2016), Efficient phosphatidylserine synthesis by a phospholipase D from Streptomyces sp. SC734 isolated from soil contaminated palm oil, Eur. J. Lipid Sci. Technol., 118, 803-813, link.
[346] Skalden, L., Peters, C., Ratz, L., Bornscheuer, U. T. (2016), Synthesis of (1R,3R)-1-amino-3-methylcyclohexane by an enzyme cascade reaction, Tetrahedron, 72, 7207-7211, link.
2011 - 2015
[345] Süss, P., Borchert, S., Hinze, J., Illner, S., von Langermann, J., Kragl, U., Wardenga, R., Bornscheuer, U.T. (2015), Chemoenzymatic sequential multistep one-pot reaction for the synthesis of (1S, 2R)-1-(methoxycarbonyl)cyclohex-4-ene-2-carboxylic acid with recombinant pig liver esterase, Org. Proc. Res. Dev., 19, 2034-2038, link.
[344] Genz, M., Vickers, C., Bergh, T. v.d., Joosten, H.J., Dörr, M., Höhne, M., Bornscheuer, U.T. (2015), Alteration of the donor/acceptor spectrum of the (S)-amine transaminase from Vibrio fluvialis, Int. J. Mol. Sci., 6, 26953-26963, link.
[343] Isupov, M.N., Schröder, E., Gibson, R.P., Beecher, J., Donadio, G., Saneei, V., Dcunha, S.A., McGhie, E.J., Sayer, C., Davenport, C.F., Lau, P.C., Hasegawa, Y., Iwaki, H., Kadow, M., Balke, K., Bornscheuer, U.T., Bourenkov, G., Littlechild, J.A. (2015), The oxygenating constituent of 3,6-diketocamphane monooxygenase from the CAM plasmid of Pseudomonas putida: the first crystal structure of type II Baeyer-Villiger monooxygenase, Acta Cryst., Sect. D., D71, 2344–2353, link.
[342] Schmidt, S., Genz, M., Balke, K., Bornscheuer, U.T. (2015), The effect of disulfide bond introduction and related Cys/Ser mutations on the stability of a cyclohexanone monooxygenase, J. Biotechnol., 214, 199-211, link.
[341] Wang, Y., Guo, S., Xu, J., Pavlidis, I., Lan, D., Bornscheuer, U., Liu, J. (2015), Structure of product-bound SMG1 lipase: active site gating implications, FEBS J., 282, 4538-4547, link.
[340] Santos-Aberturas, J., Dörr, M., Waldo, G.S., Bornscheuer, U.T. (2015), In-depth high-throughput screening of protein engineering libraries by split-GFP direct crude cell extract data normalization, Chem. Biol., 22, 1406-1414, link.
[339] Smith, M.E., Fibinger, M.P.C., Bornscheuer, U.T., Masterson, D. (2015), An investigation of the interaction of co-solvent with substrates in the pig liver esterase catalyzed hydrolysis of malonate esters, ChemCatChem, 7, 3179-3185, link.
[338] Schmidt, S., Büchsenschütz, H.C., Scherkus, C., Liese, A., Gröger, H., Bornscheuer, U.T. (2015), Biocatalytic access to chiral polyesters by an artificial enzyme cascade synthesis, ChemCatChem, 7, 3951-3955, link.
[337] Müller, J., Sowa, M.A., Dörr, M., Bornscheuer, U.T. (2015), The acyltransferase activity of lipase CAL-A allows efficient fatty acid esters formation from plant oil even in an aqueous environment, Eur. J. Lipid Sci. Technol., 117, 1903-1907, link.
[336] Bornscheuer, U.T., Nielsen, A.T. (2015), Interdisciplinary concepts for modern biotechnological production of biochemicals and biofuels, Curr. Opin. Biotechnol., 35, 133-134, link.
[335] Bornscheuer, U.T. (2015), Discovery, engineering and application of enzymes in lipid modification, INFORM, 26, 468-471.
[334] Müller, J., Sowa, M.A., Fredrich, B., Brundiek, H., Bornscheuer, U.T. (2015), Enhancing the acyltransferase activity of Candida antarctica lipase A by rational design, ChemBioChem, 16, 1791-1796, link.
[333] Lan, D., Popowicz, G.M., Pavlidis, I.V., Zhou P., Bornscheuer, U.T., Wang, Y. (2015) Conversion of a mono- and diacylglycerol lipase into a triacylglycerol lipase by protein engineering, ChemBioChem, 16, 1431-1434, link.
[332] Fibinger, M.P.C., Davids, T., Böttcher, D., Bornscheuer, U.T. (2015), A selection assay for haloalkane dehalogenase activity based on toxic substrates, Appl. Microb. Biotechnol. 99, 8955-8962, link.
[331] Kohls, H., Anderson, M., Dickerhoff, J., Weisz, K., Córdova, A., Berglund, P., Brundiek, H., Bornscheuer, U.T., Höhne, M. (2015), Selective access to all four diastereomers of an 1,3-amino alcohol by combination of a keto reductase- and an amine transaminase-catalysed reaction, Adv. Synth. Catal., 357, 1808-1814, link.
[330] Thomsen, M., Tuukkanen, A., Dickerhoff, J., Palm, G.J., Kratzat, H. Svergun, D., Weisz, K., Bornscheuer, U.T., Hinrichs, W. (2015), Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase, Acta Cryst. Sect. D., D71, 907-917, link.
[329] Dörr, M., Böttcher, D., Hummel, A., Höhne, M., Bornscheuer, U.T. (2015) Eine Roboterplattform für das Hochdurchsatz-Screening von Biokatalysatoren, Biospektrum, 21, 230-231, link.
[328] Skalden, L., Peters, C., Dickerhoff, J., Nobili, A., Joosten, H.J., Weisz, K., Höhne,M., Bornscheuer, U.T. (2015) Two subtle amino acid changes in a transaminase substantially enhance or invert enantiopreference in cascade syntheses, ChemBioChem, 16, 1041-1045, link.
[327] Nobili, A., Tao, Y, Pavlidis, I.V., van den Bergh, T., Joosten, H.-J., Tan, T., Bornscheuer, U.T. (2015), Simultaneous use of in silico design and a correlated mutation network as a tool to efficiently guide enzyme engineering, ChemBioChem, 16, 805-810, link.
[326] Jeon, Y., Baek, A.H., Bornscheuer, U.T., Park, J.B. (2015), Enzyme fusion for whole-cell biotransformation of long chain sec-alcohols into esters, Appl. Microbiol. Biotechnol., 99, 6267-6275, link.
[325] Bornscheuer, U.T. (2015) Protein-Engineering in der Biokatalyse, Biospektrum, 21, 112-113, link.
[324] Steffen-Munsberg, F., Vickers, C., Kohls, H., Land, H., Mallin, H., Nobili, A., Skalden, L., van den Bergh, T., Joosten, H.-J., Berglund, P., Höhne, M., Bornscheuer, U.T. (2015) Bioinformatic analysis of a PLP-dependent enzyme superfamily suitable for biocatalytic applications, Biotechnol. Adv., 33, 566-604, link.
[323] Nobili, A., Steffen-Munsberg, F., Kohls, H., Trentin, I., Schulzke, C., Höhne, M., Bornscheuer, U.T. (2015) Engineering the active site of the amine-transaminase from Vibrio fluvialis for the asymmetric synthesis of aryl-alkyl amines and amino alcohols, ChemCatChem, 7, 757-760, link.
[322] Muschiol, J., Peters, C., Oberleitner, N., Mihovilovic, M.D., Bornscheuer, U.T., Rudroff, F. (2015) Cascade catalysis – strategies and challenges en route to preparative synthetic biology, Chem. Comm., 51, 5798-5811,link.
[321] Schmidt, S., Scherkus, C., Muschiol, J., Menyes, U., Winkler, T., Hummel, W., Gröger, H., Liese, A., Herz, H.G., Bornscheuer, U.T. (2015), An enzyme cascade synthesis of ε-caprolactone and its oligomers, Angew. Chem. Int. Ed., 54, 2784-2787, link; Eine Enzymkaskade zur Synthese von ε-Caprolacton und dessen Oligomeren, Angew. Chem., 127, 2825-2828, link.
[320] Oh, H.J., Kim, S.U., Song, J.W., Lee, J.H., Kang, W.R., Jo, Y.S., Kim, K.R., Bornscheuer, U.T., Oh, D.K., Park, J.B. (2015), Biotransformation of linoleic acid into hydroxy fatty acids and carboxylic acids using a linoleate double bond hydratase as key enzyme, Adv. Synth. Catal., 357, 408-416, link.
[319] Santos-Aberturas, J., Engel, J., Dickerhoff, J., Dörr, M., Rudroff, F., Weisz, K., Bornscheuer U.T. (2015), Exploration of the substrate promiscuity of biosynthetic tailoring enzymes as a new source of structural diversity for polyene macrolide antifungals, ChemCatChem, 7, 490-500, link.
[318] Skalden, L., Thomsen, M., Höhne, M., Bornscheuer, U.T., Hinrichs, H. (2015), Structural and biochemical characterization of the dual substrate recognition of the (R )-selective amine transaminase from Aspergillus fumigatus, FEBS J., 282, 407-415, link.
[317] Schwenteit, J.M., Weber, B., Milton, D.L., Bornscheuer, U.T., Gudmundsdottir. B.K. (2015), Construction of Aeromonas salmonicida subsp. achromogenes AsaP1-toxoid strains and study of their ability to induce immunity in Arctic charr (Salvelinus alpinus, L.), J. Fish Disease, 38, 891-900, link.
[316] Lee, Y.A., Jeon, E.Y., Song, J.W., Bornscheuer, U.T., Park, J.B. (2014), Engineering the substrate binding domain of an esterase enhances hydrolytic activity towards fatty acid esters, Proc. Biochem., 49, 2101-2016, link.
[315] Weiß, M.S., Pavlidis, I.V., Vickers, C., Höhne, M., Bornscheuer, U.T. (2014), A glycine oxidase based high-throughput solid-phase-assay for substrate profiling and directed evolution of (R )- and (S )-selective amine transaminases, Anal. Chem, 86, 11847-11853, link.
[314] Beier, A., Hahn, V., Bornscheuer, U.T., Schauer, F. (2014) Metabolism of alkenes and ketones by Candida maltosa and related yeasts, AMB Express, 4, 75, link.
[313] Bornscheuer, U.T., Buchholz, K., Seibel, J. (2014), Enzymatic degradation of (ligno-)celluloses, Angew. Chem. Int. Ed., 53, 10876-10893, link; Bornscheuer, U.T., Buchholz, K., Seibel, J. (2014), Enzymatischer Abbau von (Ligno-)cellulose, Angew. Chem., 126, 11054-11073, link.
[312] Süss, P., Illner, S., von Langermann, J., Borchert, S., Wardenga, R. Bornscheuer, U.T., Kragl, U. (2014), Scale-up of a recombinant pig liver esterase-catalyzed desymmetrization of dimethyl-cyclohex-4-ene-cis-1,2-dicarboxylate, Org. Proc. Res. Dev., 18, 897-903, link.
[311] Bornscheuer, U.T. (2014) Enzymes in lipid modification: past achievements and current trends, Eur. J. Lipid Sci. Technol., 116, 1322-1331, link.
[310] Mallin, H., Höhne, M., Bornscheuer, U.T. (2014), Immobilization of (R )- and (S )-amine transaminases on chitosan support and their application for amine synthesis using isopropylamine as donor, J. Biotechnol., 191, 32-37, link.
[309] Hidalgo, A., Schließmann, A., Bornscheuer, U.T (2014), One-pot, simple methodology for cassette randomization and recombination for focused directed evolution (OSCARR), Methods Mol. Biol., 1179, 207-212, link.
[308] Song, J.W., Lee, J.H., Bornscheuer, U.T., Park, J.B. (2014), Microbial synthesis of medium chain α,ω-dicarboxylic acids and ω-aminocarboxylic acids from renewable long chain fatty acids, Adv. Synth. Catal., 356, 1782-1788, link.
[307] Oberleitner, N., Peters, C., Rudroff, F., Bornscheuer, U.T., Mihovilovic, M.D. (2014), In vitro characterization of an enzymatic redox cascade composed of an alcohol dehydrogenase, enoate reductases and a Baeyer-Villiger monooxygenase, J. Biotechnol., 192, 393-399, link.
[306] Pavlidis, I.V. Patila, M. Bornscheuer, U.T., Gournis, D., Stamatis, H. (2014), Graphene-based nanobiocatalytic systems: recent advances and future prospects, Trends Biotechnol., 32, 312-320, link.
[305] Beller, M., Bornscheuer, U.T. (2014), CO2 fixation through hydrogenation by chemical or enzymatic methods, Angew. Chem. Int. Ed., 53, 4527-4528, link; Fixierung von CO2 durch Hydrierung mit chemischen oder enzymatischen Methoden, Angew. Chem., 126, 4615-4617, link.
[304] Peters, C., Kölzsch, R., Kadow, M., Skalden, L., Rudroff, F., Mihovilovic, M.D., Bornscheuer, U.T. (2014), Identification, characterization and application of three enoate reductases from P. putida in in vitro enzyme cascade reactions, ChemCatChem, 6, 1021-1027, link.
[303] Thomsen, M., Skalden, L., Palm, G.J., Höhne, M., Bornscheuer, U.T., Hinrichs, W. (2014), Crystallographic characterization of the (R)-selective amine transaminase from Aspergillus fumigatus, Acta Crystal. Section D., 70, 1086-1093, link.
[302] Gall, M., Thomsen, M., Peters, C., Pavlidis, I.V., Jonczyk, P., Grünert, P.P., Beutel, S., Scheper, T., Gross, E., Backes, M., Ley, J.P., Hilmer, J.M., Krammer, G., Geissler, T., Palm, G., Hinrichs, W., Bornscheuer, U.T. (2014), Enzymatic conversion of flavonoids using bacterial chalcone isomerase and enoate reductase, Angew. Chem. Int. Ed., 53, 1439-1442, link; Enzymatische Umsetzung von Flavonoiden mit einer bakteriellen Chalconisomerase und einer Enoatreduktase, Angew. Chem., 126, 1463-1466, link.
[301] Kadow, M., Balke, K., Willetts, A., Bornscheuer, U.T., Bäckvall, J.E. (2014), Functional assembly of camphor converting two-component Baeyer-Villiger monooxygenases with a flavin reductase from E. coli, Appl. Microb. Biotechnol., 98, 3975-3986, link.
[300] Müller, J., Fredrich, B., Kohlmann, C., Maksym, L., Bornscheuer, U.T. (2014), A high-throughput assay for the determination of acyltransferase activity of lipase CAL-A, Eur. J. Lipid Sci. Technol., 116, 232–236, link.
[299] Hackenschmidt, S., Moldenhauer, E.V., Behrens, G.A., Gand, M., Pavlidis, I.V., Bornscheuer, U.T. (2014), Enhancement of promiscuous amidase activity of a Bacillus subtilis esterase by formation of a π-π-network, ChemCatChem, 6, 1015-1020,link.
[298] Gall, M., Nobili, A., Pavlidis, I.V., Bornscheuer, U.T. (2014), Improved thermostability of a Bacillus subtilis esterase by domain exchange, Appl. Microb. Biotechnol., 98, 1719-1726, link.
[297] Höhne, M., Bornscheuer, U.T. (2014), Protein engineering from 'scratch' is maturing, Angew. Chem. Int. Ed., 53, 1200-1202, link; Protein engineering aus dem "Nichts" wird praktikabel, Angew. Chem., 126, 1222-1224, link.
[296] Thomsen, M. Skalden, L., Palm, G., Höhne, M., Bornscheuer, U.T., Hinrichs, W. (2013), Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus, Acta Cryst. Sect. F., 69, 1415-1417, link.
[295] Leipold F., Rudroff, F., Mihovilovic, M.D., Bornscheuer, U.T. (2013), The steroid monooxygenase from Rhodococcus rhodochrous – a versatile biocatalyst, Tetrahedron:Asymmetry, 24, 1620-1624, link.
[294] Mallin, H., Muschiol, J., Byström, E., Bornscheuer, U.T. (2013), Efficient biocatalysis with immobilized enzymes or encapsulated whole cell microorganism using the SpinChem reactor system, ChemCatChem, 5, 3529-3532, link.
[293] Oberleitner, N., Peters, C., Muschiol, J. , Kadow, M., Saß, S., Bayer, T., Schaaf, P., Iqbal, N., Rudroff, F., Mihovilovic, M.D., Bornscheuer, U.T. (2013), An enzymatic toolbox for cascade reactions: a showcase for an in vivo redox sequence in asymmetric synthesis, ChemCatChem, 5, 3524-3528, link.
[292] Schwenteit, J.M., Breithaupt, A., Teifke, J.P., Koppang, E.O., Bornscheuer, U.T., Fischer, U., Gudmundsdottir. B.K. (2013), Innate and adaptive immune response of Arctic charr (Salvelinus alpinus, L.) during bacterial infection with A. salmonicida subsp. achromogenes and the effect of the AsaP1 toxin, Fish Shellfish Immunol., 35, 866-873, link.
[291] Bornscheuer, U.T. (2013), Protein engineering as a tool for the development of novel bioproduction systems, Adv. Biochem. Eng. / Biotechnol., 137, 25-40, link.
[290] Adamczak, M., Bornscheuer, U.T. (2013), Application of medium engineering in the synthesis of structured triacylglycerols from evening primrose oil (Oenothera biennis L.), Eur. J. Lipid Sci. Technol., 115, 405-412, link.
[289] Davids, T., Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2013) Strategies for the discovery and engineering of enzymes for Biocatalysis, Curr. Opin. Chem. Biol., 17, 215-220, link.
[288] Herter, S., Michalik, D., Mikolasch, A., Schmidt, M., Wohlgemuth, R., Bornscheuer, U.T., Schauer, F. (2013), Laccase-mediated synthesis of 2-methoxy-3-methyl-5-(alkylamino)- and 3-methyl-2,5-bis(alkylamino)-[1,4]-benzoquinones, J. Mol. Catal. B: Enzymatic, 90, 91-97, link.
[287] Staudt, S., Bornscheuer, U.T., Menyes, U., Hummel, W., Gröger, H. (2013), Direct biocatalytic one-pot-transformation of cyclohexanol with molecular oxygen into ε-caprolactone, Enzyme Microb. Technol., 53, 288-292, link.
[286] Wulf, H., Mallin, H., Bornscheuer, U.T. (2013), A self-sufficient Baeyer-Villiger biocatalysis system for the synthesis of ε-caprolactone from cyclohexanol, Enzyme Microb. Technol., 53, 283-287, link.
[285] Nobili, A., Gall, M.G. Pavlidis, I.V., Thompson, M.L., Schmidt, M., Bornscheuer, U.T. (2013), Use of "small, but smart" libraries to enhance the enantioselectivity of an esterase from Bacillus stearothermophilus towards tetrahydrofuran-3-yl-acetate, FEBS J, 280, 3084-3093,link.
[284] Song, J.W., Jeon, E.Y., Song, D.H., Jang, H.Y., Bornscheuer, U.T., Oh, D.K., Park, J.B. (2013), Multistep enzymatic synthesis of long-chain α,ω-dicarboxylic and ω-hydroxycarboxylic acids from renewable fatty acids and plant oils, Angew. Chem. Int. Ed., 52, 2534-2537, link; Angew. Chem., 125, 2594-2597, link.
[283] Koudelakova, T., Chaloupkova, R., Brezovsky, J., Prokop,Z., Sebestova, E., Hesseler, M., Khabiri, M., Plevaka, M., Kulik, D., Smatanova, I.K., Rezacova, P., Ettrich, R., Bornscheuer, U.T., Damborsky, J. (2013), Engineering enzyme stability and resistance to an organic co-solvent by modification of residues in the access tunnel, Angew. Chem. Int. Ed., 52, 1959-1963, link; Angew. Chem., 125, 2013-2017, link.
[282] Bornscheuer, U.T. (2013), From commercial enzymes to biocatalysts designed by protein engineering, Synlett, 24, 150-156, link.
[281] Mallin, H., Menyes, U., Vorhaben, T., Höhne, M., Bornscheuer, U.T. (2013), Immobilization of two (R )-amine transaminases on an optimized chitosan support for the enzymatic synthesis of optically pure amines, ChemCatChem, 5, 588-593, link.
[280] Steffen-Munsberg, F., Vickers, C., Thontowi, A., Schätzle, S., Tumlirsch, T., Svedendahl Humble, M., Land, H., Berglund, P., Bornscheuer, U.T., Höhne, M. (2013), Connecting unexplored protein crystal structures to enzymatic function, ChemCatChem, 5, 150-153, link.
[279] Steffen-Munsberg, F., Vickers, C., Thontowi, A., Schätzle, S., Meinhardt, T., Svedendahl Humble, M., Land, H., Berglund, P., Bornscheuer, U.T., Höhne, M. (2013), Revealing the structural basis of promiscuous amine transaminase activity, ChemCatChem, 5, 154-157, link.
[278] Schwenteit, J., Bogdanovic, X., Fridjonsson, O.H., Bornscheuer, U.T., Hinrichs, W. Gudmundsdottir. B.K. (2013), Toxoid construction of AsaP1, a lethal toxic aspzincin metalloendopeptidase of Aeromonas salmonicida subsp. achromogenes, and studies of its activity and processing, Vet. Microbiol., link.
[277] Bornscheuer, U.T. (2013), Enzymes in lipid modification: from classical biocatalysis with commercial enzymes to advanced protein engineering tools, Oléagineux Corps gras Lipides, 45-49, link.
[276] Chow, J., Krauss, U., Dall Antonia, Y. Fersini, F., Schmeisser, C., Schmid, M. Menyes, I., Bornscheuer, U.T., Lauinger, B., Bongen, P., Pietruszka, J., Eckstein, M., Thum, O., Liese, Mueller-Dieckmann, J., Jaeger, K.-E., Streit, W.R. (2012), LipS and LipT, two metagenome-derived lipolytic enzymes increase the diversity of known lipases, PLOS One, link.
[275] Kuhn, M.L., Martinez, S., Gumataotao, N., Bornscheuer, U.T., Liu, D., Holz, R.C. (2012), The Fe-type nitrile hydratase from Comamonas testosteroni Ni1 does not require an activator accessory protein for expression in Escherichia coli, Biochem. Biophys. Res. Commun., 424, 365-370, link.
[274] Wulf, H., Mallin, H., Bornscheuer, U.T. (2012), Protein engineering of a thermostable polyol-dehydrogenase, Enzyme Microb. Technol., 51, 217-224, link.
[273] Soumanou, M.M., Djenontin, S.T., Tchobo, F.P., Sohounhloue, D.C.K., Bornscheuer, U.T. (2012), Lipase-catalyzed biodiesel production from Jatropha curcas oil, Lipid Technol., 24, 158-160, link.
[272] Balke, K., Kadow, M., Mallin, H., Saß, S., Bornscheuer, U.T. (2012), Discovery, application and protein engineering of Baeyer-Villiger monooxygenases in organic synthesis, Org. Biomol. Chem., 10, 6249-6265, link.
[271] Saß, S. Kadow, M., Geitner, K. Thompson, M., Talmann, L., Böttcher, D., Schmidt, M., Bornscheuer, U.T. (2012), A high-throughput assay method to quantify Baeyer-Villiger monooxygenase activity, Tetrahedron, 68, 7575-7580, link.
[270] Hasenpusch, S., Möller, D., Bornscheuer, U.T., Langel, W. (2012), Substrate-enzyme interaction in pig liver esterase, arXiv.org, online.
[269] Oh, K.H., Nguyen, G.-S., Kim, E.Y., Kourist, R., Bornscheuer, U.T., Oh, T.-K., Yoon, J.-H. (2012), Characterization of a novel esterase isolated from intertidal flat metagenome and its tertiary alcohols synthesis, J. Mol. Catal. B: Enzym., 80, 67-73, link.
[268] Brundiek, H., Padhi, S.K., Evitt, A., Kourist, R., Bornscheuer, U.T. (2012), Altering the scissile fatty acid binding site of Candida antarctica lipase A by protein engineering for the selective hydrolysis of medium chain fatty acids, Eur. J. Lipid Sci. Technol., 114, 1148-1153, link.
[267] Pavlidis, I.V., Vorhaben, T., Gournis, D., Papadopoulos G.K., Bornscheuer, U.T., Stamatis, H. (2012), Regulation of catalytic behavior of hydrolases through interactions with functionalized carbon-based nanomaterials, J. Nanoparticle Res., 14, 842, link.
[266] Bornscheuer, U.T., Huisman, G., Kazlauskas, R.J., Lutz, S., Moore, J., Robins, K. (2012) Engineering the third wave in biocatalysis, Nature, 485, 185-194, link.
[265] Gröger, H., Asano, Y., Bornscheuer, U.T., Ogawa, J., (2012) Development of biocatalytic processes in japan and germany – from research synergies to industrial applications, Chem. Asian J., 7, 1138-1153, link.
[264] Padhi, S.K., Haas, M., Bornscheuer, U.T. (2012), Lipase-catalyzed transesterification to remove saturated monoacylglycerols from biodiesel, Eur. J. Lipid Sci. Technol., 114, 875-879, link.
[263] Smith, M.E., Banerjee, S., Shi, Y., Schmidt, M., Bornscheuer, U.T. Masterson, D.S. (2012), Investigation of the cosolvent effect on six isoenzymes of PLE in the enantioselective hydrolysis of selected a,a-disubstituted malonate esters, ChemCatChem, 4, 472-475, link.
[262] Schließmann, S., Schmidt, M., Torres, L.L., Gámez, J.A., Silva-Martín, N., Hermoso, J.A., Berenguer, J., Bornscheuer, U.T., Hidalgo, A. (2012) Promiscuous lactamase activity in an esterase originates from electronic, steric and mechanistic determinants, Org. Biomol. Chem., 10, 3388-3392, link.
[261] Brundiek, H., Sass, S., Evitt, A., Kourist, R., Bornscheuer, U.T. (2012), The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans fatty acid selectivity, Appl. Microb. Biotechnol., 94, 141-150; erratum: 94, 285, link.
[260] Gerstenbruch, S., Wulf, H., Mußmann, N., O'Connell, T., Maurer, K.-H., Bornscheuer, UT. (2012), Directed evolution of an alditol oxidase from Streptomyces coelicolor for enhanced oxidative activity towards glycerol, Appl. Microb. Biotechnol., 96, 1243-1252, link.
[259] Kadow, M., Loschinski, K., Saß, S., Schmidt, M., Bornscheuer, U.T. (2012), Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli and biochemical characterization, Appl. Microb. Biotechnol., 96, 419-429, link.
[258] Pavlidis, I.V., Vorhaben, T., Tsoufis, T., Rudolf, P., Bornscheuer, U.T., Gournis, D., Stamatis, H. (2012) Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials, Biores. Technol., 115, 164-171, link.
[257] Brundiek, H.B., Evitt, A.S., Kourist, R., Bornscheuer, U.T. (2012), Creation of a lipase highly selective for trans fatty acid by protein engineering, Angew. Chem. Int. Ed., 51, 412-414, link; Erzeugung einer für trans-Fettsäuren hochselektiven Lipase durch Protein-Engineering, Angew. Chem., 124, 425-428, link.
[256] Leipold, F., Wardenga, R., Bornscheuer, U.T. (2012), Cloning, expression and characterisation of an eukaryotic cycloalkanone monooxygenase from Cylindrocarpon radicicola ATCC 11011, Appl. Microb. Biotechnol., 94, 705-717, link.
[255] Rehdorf, J., Behrens, G.A., Nguyen, G.-S., Kourist, R., Bornscheuer, U.T. (2012), Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols, Appl. Microb. Biotechnol., 93, 1119-1126, link.
[254] Wulf, H., Perzborn, M., Sievers, G., Scholz, F., Bornscheuer, U.T. (2012), Kinetic resolution of glyceraldehyde using an aldehyde dehydrogenase from Deinococcus geothermalis DSM11300 combined with electrochemical cofactor recycling, J. Mol. Catal. B: Enzymatic, 74, 144-150, link.
[253] Vorhaben, T., Häckel, M., Bornscheuer, U.T., Menyes, U. (2011) Plasmagestützte Immobilisierung von Enzymen, Nachrichten Chemie, 59, 1147-1149.
[252] Jonczyk, P., Schmidt, A., Bice, I., Lück, M., Gross, E., Hilmer, J.M., Bornscheuer, U., Beutel, S., Scheper, T. (2011), Strikt anaerobe batch-Kultivierung von Eubacterium ramulus in neuartigem Einweg-Beutelreaktorsystem, Chem. Ing. Techn., 83, 2147-2152, link.
[251] Bornscheuer, U.T., Kazlauskas, R.J. (2011), Survey of protein engineering strategies, Curr. Prot. Prot. Sci., 66, 26.7.1-26.7.14, link.
[250] Herter, S., Mikolasch, A., Michalik, D., Hammer, E., Schauer, F., Bornscheuer, U.T., Schmidt M. (2011), C-N coupling of 3-methylcatechol with primary amines using native and recombinant laccases from Trametes versicolor and Pycnoporus cinnabarinus, Tetrahedron,67, 9311-9321, link.
[249] Behrens, G.A., Hummel, A., Padhi, S.K., Schätzle, S., Bornscheuer, U.T. (2011) Discovery and protein engineering of biocatalysts for organic synthesis, Adv. Synth. Catal., 353, 2191-2215, link.
[248] Schätzle, S., Steffen-Munsberg, F., Thontowi, A., Höhne, M., Robins, K., Bornscheuer, U.T. (2011), Enzymatic asymmetric synthesis of enantiomerically pure aliphatic, aromatic and arylaliphatic amines with (R )- selective amine transaminases, Adv. Synth. Catal., 353, 2439-2445, link.
[247] Fernández-Àlvaro, E., Snajdrova, R., Jochens, H., Davids, T., Böttcher, D., Bornscheuer, U.T. (2011), A combination of in vivo selection and cell sorting for the identification of enantioselective biocatalysts, Angew. Chem. Int. Ed., 50, 8584-8587, link; Eine Kombination aus In-vivo-Selektion und Zellsortierung zur Identifizierung enantioselektiver Biokatalysatoren, Angew. Chem., 123, 8742-8746, link.
[246] Kadow, M., Saß, S., Schmidt, M., Bornscheuer, U.T. (2011), Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007, AMB Express, link.
[245] Kourist, R., Bornscheuer, U.T. (2011), Biocatalytic synthesis of optically active tertiary alcohols, Appl. Microb. Biotechnol., 91, 505-517, link.
[244] Palm, G.J., Fernández-Alvaro, E., Bogdanovic, X., Bartsch, S., Sczodrok, J., Böttcher, D., Singh, R.K., Atomi, H., Bornscheuer, U.T., Hinrichs, W. (2011), The crystal structure of an esterase from the hyperthermophilic microorganism Pyrobaculum calidifontis VA1 supports explanation of its enantioselectivity, Appl. Microbiol. Biotechnol., 91, 1061-1072, link.
[243] Hesseler, M., Bogdanovic, X., Hidalgo, A., Berenguer, J., Palm, G., Hinrichs, W., Bornscheuer, U.T. (2011), Cloning, functional expression, biochemical characterization and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1, Appl. Microb. Biotechnol., 91, 1049-1060, link.
[242] Jochens, H., Hesseler, M., Stiba, K., Padhi, S.K., Kazlauskas R.J., Bornscheuer, U.T. (2011), Protein engineering of α/β-hydrolase fold enzymes, ChemBioChem, 12, 1508-1517, link.
[241] Ni, Y., Li, C.X., Zhang, J., Shen, N.-D., Bornscheuer, U.T., Xu, J.H. (2011), Efficient reduction of ethyl 2-oxo-4-phenylbutyrate at 620 g/L by a bacterial reductase with broad substrate spectrum, Adv. Synth. Catal., 353, 1213-1217, link.
[240] Cassimjee, K.E, Kourist, R., Lindberg, D., Wittrup-Larsen, M., Nguyen, H.T., Widersten, M., Bornscheuer, U.T., Berglund, P. (2011), One-step enzyme extraction and immobilisation for biocatalysis applications, Biotechnol. J., 6, 463-469, link.
[239] Evitt, A., Bornscheuer, U.T. (2011), Lipase CAL-B does not catalyze a promiscuous decarboxylative aldol addition or Knoevenagel reaction, Green Chem., 13, 1141-1142, link.
[238] Biermann, U., Bornscheuer, U.T., Meier, M.A.R., Metzger, J.O., Schäfer, H.J. (2011) New developments for the chemical utilization of oils and fats as renewable raw materials, Angew. Chem. Int. Ed., 50, 3854-3871, link; Neue Entwicklungen zur stofflichen Nutzung von Fetten und Ölen als nachwachsende Rohstoffe, Angew. Chem., 123, 3938-3956, link.
[237] Nguyen, G.S., Thompson, M.L., Grogan, G., Bornscheuer, U.T., Kourist, R. (2011), Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining, J. Mol. Catal. B: Enzymatic, 70, 88-94, link.
[236] Herter, S., Nguyen, G.S., Thompson, M.L., Steffen-Munsberg, F., Schauer, F., Bornscheuer, U.T., Kourist, R. (2011), Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries, Appl. Microbiol. Biotechnol., 90, 929-939, link.
[235] Hasenpusch, D., Bornscheuer, U.T., Langel, W. (2011), Simulation on the structure of pig liver esterase, J. Mol. Model., 17, 1493-1506, link.
[234] Schwenteit, J., Gram, L., Nielsen, K.F., Fridjonsson, O.H., Bornscheuer, U.T., Givskov, M., Gudmundsdottir, B.K. (2011), Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence, Vet. Microbiol., 147, 389-397, link.
2006 - 2010
[233] Bartsch, S., Bornscheuer, U.T. (2010) Mutational analysis of phenylalanine ammonia lyase to improve reaction rates for various substrates, Protein Eng. Des. Sel., 23, 929-933, link.
[232] Höhne, M., Schätzle, S., Jochens, H., Robins, K., Bornscheuer, U.T. (2010) Rational assignment of key motifs for function guides in silico enzyme identification, Nature Chem. Biol., 6, 807-813, link (Highlighted recently "Best design concept" by Nat. Chem. Biol.).
[231] Jochens, H., Aerts, D., Bornscheuer, U.T. (2010) Thermostabilization of an esterase by alignment-guided focused directed evolution, Protein Eng. Des. Sel., 23, 903-909, link.
[230] Geitner, K., Rehdorf, J., Snajdrova, R., Bornscheuer, U.T. (2010) Scale-up of Baeyer-Villiger monooxygenase-catalyzed synthesis of enantiopure compounds, Appl. Microb. Biotechnol., 88, 1087-1093, link.
[229] Böttcher, D., Schmidt, M., Bornscheuer, U.T. (2010), Screens for active and stereoselective hydrolytic enzymes. Methods Mol. Biol., 668, 169-176, link.
[228] Rehdorf, J., Mihovilovic, M.D., Fraaije, M.W., Bornscheuer, U.T. (2010), Enzymatic synthesis of enantiomerically pure ß-amino ketones, ß-amino esters and ß-amino alcohols with Baeyer-Villiger-monooxygenases, Chem. Eur. J., 16, 9525-9535, link.
[227] Jochens, H., Bornscheuer, U.T. (2010), Natural diversity to guide focused directed evolution, ChemBioChem, 11, 1861-1866, link.
[226] Vorhaben, T., Böttcher, D., Jasinski, D., Brüser, V., Menyes, U., Schröder, K., Bornscheuer, U.T. (2010) Plasma-modified polypropylene as carrier for the immobilization of Candida antarctica lipase B and Pyrobaculum calidifontis esterase, ChemCatChem, 2, 992-996, link.
[225] Bassegoda, A., Nguyen, G.S., Kourist, R., Schmidt, M., Diaz, P., Bornscheuer, U.T. (2010), Rational protein design of Paenibacillus barcinonensis esterase EstA for kinetic resolution of tertiary alcohols, ChemCatChem, 2, 962-967, link.
[224] Kourist, R., Jochens, H., Bartsch, S., Kuipers, R., Padhi, S.K., Gall, M., Böttcher, D., Joosten, H.-J., Bornscheuer, U.T. (2010), The α/β-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering, ChemBioChem, 11, 1635-1643, link.
[223] Bornscheuer, U.T. (2010), The first artificial cell – arevolutionary step in synthetic biology?, Angew. Chem. Int. Ed., 49, 5228-5230, link; Bornscheuer, U.T. (2010), Die erste künstliche Zelle – Ein revolutionärer Schritt für die synthetische Biologie?, Angew. Chem., 122, 5357-5359, link.
[222] Bogdanovic, X., Hesseler, M., Palm, G.J., Bornscheuer, U.T., Hinrichs, W. (2010), Crystallization and preliminary X-ray diffraction studies on the putative haloalkane dehalogenase DPpA from Plesiocystis pacifica SIR-I, Acta Cryst. Sect. F: Struct. Biol. Cryst. Commun., 66, 828-830.
[221] Rehdorf, J., Mihovilovic, M.D., Bornscheuer, U.T. (2010), Exploiting the regioselectivity of Baeyer-Villiger-monooxygenases for the formation of ß-amino acids and ß-amino alcohols, Angew. Chem. Int. Ed., 49, 4506-4508, link; Rehdorf, J., Mihovilovic, M.D., Bornscheuer, U.T. (2010), Durch Nutzen der Regioselektivität von Baeyer-Villiger-Monooxygenasen zu ß-Aminosäuren und ß-Aminoalkoholen, Angew. Chem., 122, 4609-4611, link.
[220] Bornscheuer, U.T., Hesseler, M. (2010), Enzymatic removal of 3-monochloropropane diol (3-MCPD) and its esters from oils, Eur. J. Lipid Sci. Technol., 112, 552-556, link.
[219] Kuipers, R.K., Joosten, H.J., van Berkel, W.J.H., Leferink, N.G.H., Rooijen, E., Ittmann, E., van Zimmeren, F., Jochens, H., Bornscheuer, U., Vriend, G., Martins dos Santos, V.A.P., Schaap, P.J. (2010), 3DM: systematic analysis of heterogeneous super-family data to discover protein functionalities, Proteins, 78, 2101-2113, link.
[218] Gall, M., Kourist, R., Schmidt, M., Bornscheuer, U.T. (2010), The role of the GGG(A)X-motif on enantioselectivity of pig liver esterase towards tertiary alcohols, Biocat. Biotransform, 28, 201-208, link.
[217] Nguyen, G.S., Kourist, R., Paravidino, M., Hummel, A., Rehdorf, J., Orru, R.V.A., Hanefeld, U., Bornscheuer, U.T. (2010), An enzymatic toolbox for the kinetic resolution of 2-(pyridin-x-yl)but-3-yn-2-ols and tertiary cyanohydrins, Eur. J. Org. Chem., 2753-2758, link.
[216] Theurer, M., Fischer, P., Baro, A., Nguyen, G.S., Kourist, R., Bornscheuer, U.T., Laschat, S. (2010), Formation of chiral tertiary homoallylic alcohols via Evans aldol reaction or enzymatic resolution and their influence on the Sharpless asymmetric dihydroxylation, Tetrahedron, 66, 3814-3823, link.
[215] Wittrup-Larsen, M., Zielinska, D.F., Martinelle, M., Hidalgo, A., Jensen, L.J., Bornscheuer, U.T., Hult, K. (2010), Suppression of water as a nucleophile in Candida antarctica lipase B catalysis, ChemBioChem, 11, 796-801, link.
[214] Schätzle, S., Höhne, M., Robins, K., Bornscheuer U.T. (2010), A conductometric method for the rapid characterization of the substrate specificity of amine-transaminases, Anal. Chem.,82, 2082-2086, link.
[213] Böttcher, D., Bornscheuer, U.T. (2010) Protein engineering of microbial enzymes, Curr. Opin. Microbiol., 13, 274-282, link.
[212] Brüsehaber, E., Schwiebs, A., Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2010), Production of pig liver esterase in batch fermentation of E. coli Origami, Appl. Microb. Biotechnol., 86, 1337-1344, link.
[211] Rzeznicka, K., Schätzle, S., Böttcher, D., Klein, J., Bornscheuer, U.T. (2010), Cloning and functional expression of a nitrile hydratase (NHase) from Rhodococcus equi TG328-2 in Escherichia coli, its purification and biochemical characterization, Appl. Microbiol. Biotechnol., 85, 1417-1425, link.
[210] Wardenga, R., Lindner, H.A., Hollmann, F., Thum, O., Bornscheuer, U.T. (2010) Increasing the synthesis/ hydrolysis ratio of aminoacylase-1 by site-directed mutagenesis, Biochimie, 92, 102-109, link.
[209] Kourist, R., Brundiek, H., Bornscheuer, U.T. (2010), Protein engineering and discovery of lipases, Eur. J. Lipid Sci. Technol., 112, 64-74, link.
[208] Fernandez-Alvaro, E., Kourist, R., Winter, J., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Jäger, K.E., Streit, W. Bornscheuer, U.T. (2010), Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases, Microb. Biotechnol., 3, 59-64, link.
[207] Bornscheuer, U.T. (2009) Modern tools in the discovery and design of biocatalysts, Enzyme Engineering News Japan, 62, 5-14.
[206] Brüsehaber, E., Böttcher, D., Bornscheuer, U.T. (2009), Insights into the physiological role of pig liver esterase: Isoenzymes show differences in the demethylation of prenylated proteins, Bioorg. Med. Chem., 17, 7878-7883, link.
[205] Schließmann, A., Hidalgo, A., Berenguer, J., Bornscheuer, U.T. (2009) Increased enantioselectivity by engineering bottleneck mutants in an esterase from Pseudomonas fluorescens, ChemBioChem, 10, 2920-2923, link.
[204] Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2009), Protein engineering of carboxyl esterases by rational design and directed evolution, Protein Peptide Lett., 16, 1162-1171.
[203] Thodi, K., Barbayianni, E., Fotakopoulou, I., Bornscheuer, U.T., Constantinou-Kokotou, V., Moutevelis-Minakakis, P., Kokotos, G. (2009), Study of the removal of allyl esters by Candida antarctica lipase B (CAL-B) and pig liver esterase (PLE), J. Mol. Catal. B: Enzymatic, 61, 241-246, link.
[202] Schätzle, S., Höhne, M., Redestad, E., Robins, K., Bornscheuer U.T. (2009), A rapid and sensitive kinetic assay for characterization of omega-transaminases, Anal. Chem, 81, 8244-8248, link.
[201] Barbayianni, E., Kokotos, C.G., Bartsch, S., Drakou, C., Bornscheuer, U.T., Kokotos, G. (2009), Bacillus subtilisesterase (BS2) and its double mutant have different selectivity in the removal of carboxyl protecting groups, Adv. Synth. Catal., 351, 2325-2332, link.
[200] Adamczak, M., Bednarski, W., Bornscheuer, U.T. (2009), The application of biotechnological methods for synthesis of biodiesel, Eur. J. Lipid Sci. Technol., 111, 808-813, link.
[199] Kazlauskas, R.J., Bornscheuer U.T. (2009), Finding better protein engineering strategies, Nat. Chem. Biol., 5, 526-529, link.
[198] Royter, M., Schmidt, M., Elend, C., Höbenreich, H., Schäfer, T., Bornscheuer, U.T., Antranikian, G. (2009), Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis, Extremophiles, 13, 769-783, link.
[197] Höhne, M., Bornscheuer, U.T. (2009), Biocatalytic routes to optically active amines, ChemCatChem, 1, 42-51, link.
[196] Rehdorf, J., Lengar, A., Bornscheuer, U.T., Mihovilovic, M.D. (2009), Kinetic resolution of arylaliphatic acyclic ß-hydroxyketones by recombinant whole-cell Baeyer-Villiger monooxygenases – formation of enantiocomplementary regioisomeric esters, Bioorg. Med. Chem. Lett.,19, 3739-3743 link.
[195] Wiggers, M., Holt, J., Kourist, R., Bartsch, S., Arends, I.W.C.E., Minnaard, A.J., Bornscheuer, U.T., Hanefeld, U. (2009), Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols, J. Mol. Catal. B: Enzymatic, 60, 82-86, link.
[194] Kourist, R., Höhne, M., Bornscheuer, U.T. (2009), Protein Design: Im Spannungsfeld zwischen gerichteter Evolution und rationalem Design, Chemie in unserer Zeit, 43, 132-142, link.
[193] Bartsch, S., Bornscheuer, U.T. (2009), A single residue influences the reaction mechanism of ammonia lyases and mutases, Angew. Chem. Int. Ed., 48, 3362-3365, link; Corrigendum, Angew. Chem. Int. Ed., 49, 3860 link. Einfluss einer einzelnen Aminosäure auf den Reaktionsmechanismus von Ammonium-Lyasen und -Mutasen, Angew. Chem., 121, 3412-3415, link; Corrigendum, Angew. Chem., 122, 3951, link.
[192] Jochens, H., Stiba, K., Savile, C., Fujii, R., Yu, J.-G., Gerassenkov, T., Kazlauskas, R.J., Bornscheuer, U.T. (2009), Converting an esterase into an epoxide hydrolase, Angew. Chem. Int. Ed., 48, 3532-3535, link; Umwandlung einer Esterase in eine Epoxidhydrolase, Angew. Chem., 121, 3584-3587, link.
[191] Rehdorf, J., Zimmer, C., Bornscheuer, U.T. (2009), Cloning, expression and biocatalytic investigation of 4-hydroxyacetophenone monooxygenase from Pseudomonas putida JD1, Appl. Environm. Microbiol., 75, 3106-3114, link.
[190] Rachinskiy, K., Schultz, H, Boy, M., Bornscheuer, U.T., Büchs, J. (2009), Enzyme test bench: a high-throughput enzyme characterization technique including the long term stability, Biotechnol. Bioeng.103, 302-322, link.
[189] Krause, P., Hilterhaus, L., Fieg, G., Liese, A., Bornscheuer, U.T. (2009), Chemically and enzymatically catalyzed synthesis of C6-C10 alkyl benzoates, Eur. J. Lipid Sci. Technol., 111, 194-201, link.
[188] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2009), Improving ascorbyl oleate synthesis by application of ionic liquids and water activity control by salt hydrates,Proc. Biochemistry, 44, 257-261, link.
[187] Wardenga, R., Hollmann, F., Thum, O., Bornscheuer, U.T. (2008) Functional expression of porcine aminoacylase 1 in E. coli using a codon optimized synthetic gene and molecular chaperones, Appl. Microbiol. Biotechnol., 81, 721-729, link.
[186] Heinze, B., Hoven, N., O'Connell, T., Maurer, K.H., Bartsch, S., Bornscheuer, U.T. (2008) Recovery of choline oxidase activity by in vitro recombination of individual segments, Appl. Microbiol. Biotechnol., 81, 275-282, link.
[185] Kirschner, A., Bornscheuer, U.T. (2008), Directed evolution of a Baeyer-Villiger monooxygenase to enhance enantioselectivity, Appl. Microbiol. Biotechnol., 81, 465-472, link.
[184] Bornscheuer, U.T. (2008), Alteration of lipase properties by protein engineering methods, Oléagineux Corps Gras Lipides, 15, 184-188.
[183] Wittrup-Larsen, M., Bornscheuer, U.T., Hult, K. (2008), Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems, Prot. Expr. Purif., 62, 90-97, link.
[182] Kourist, R., Nguyen, G.S., Strübing, S., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Bornscheuer, U.T. (2008), Hydrolase-catalyzed stereoselective preparation of protected a,a-dialkyl-a-hydroxycarboxylic acids, Tetrahedron: Asymmetry, 19, 1839-1843, link.
[181] Hidalgo, A., Schliessmann, A., Molina, R. Hermoso, J., Bornscheuer, U.T. (2008), An one-pot, simple methodology for cassette randomization and recombination for focused directed evolution (OSCARR), Protein Des. Eng. Sel., 21, 567-576, link.
[180] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2008), Properties and biotechnological methods to produce lipids containing conjugated linoleic acid (CLA), Eur. J. Lipid Sci. Technol., 110, 491-502, link.
[179] Höhne, M., Robins, K., Bornscheuer, U.T. (2008), A protection strategy substantially enhances rate and enantioselectivity in transaminase-catalyzed kinetic resolutions, Adv. Synth. Catal., 350, 807-812, link.
[178] Brüsehaber, E., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Bornscheuer, U.T. (2008), Asymmetric synthesis of cis-3,5-diacetoxycyclopent-1-ene using metagenome derived hydrolases, Tetrahedron: Asymmetry, 19, 730-732, link.
[177] Kourist, R., Dominguez de Maria, P., Bornscheuer, U.T. (2008), Enzymatic synthesis of optically active tertiary alcohols: Expanding the biocatalytic toolbox, ChemBioChem, 9, 491-498, link.
[176] Vahl, K., Kahlert, H., Böttcher, D., Wardenga, R., Komorsky-Lovric, S., Bornscheuer, U., Scholz, F. (2008), A potential high-throughput method for the determination of lipase activity by potentiometric FIA titrations, Anal. Chem. Acta, 44-49, link.
[175] Bartsch, S., Kourist, R., Bornscheuer, U.T. (2008), Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase, Angew. Chem. Int. Ed., 47, 1508-1511 link; Vollständige Umkehrung der Enantioselektivität gegen acetylierte tertiäre Alkohole durch eine Doppelmutation in einer Esterase aus Bacillus subtilis, Angew. Chem., 120, 1531-1534, link.
[174] Höhne, M., Kühl, S., Robins, K., Bornscheuer, U.T. (2008), Efficient asymmetric synthesis of chiral amines by combining transaminase and pyruvate decarboxylase, ChemBioChem, 9, 363-365, link.
[173] Völker, A., Kirschner, A., Bornscheuer, U.T., Altenbuchner, J. (2008), Functional expression, purification and characterization of the recombinant Baeyer-Villiger monooxygenase MekA from Pseudomonas veronii MEK700, Appl. Microbiol. Biotechnol., 77, 1251-1260, link.
[172] Bertram, M. Hildebrandt, P., Weiner, D.W., Patel, J. S., Bartnek, F., Hitchman, T., Bornscheuer, U.T. (2008), Characterization of lipases and esterases from metagenomes for lipid modification, J. Am. Oil Chem. Soc., 85, 47-53, link.
[171] Kourist, R., Bartsch, S., Fransson, L., Hult, K., Bornscheuer, U.T. (2008), Understanding promiscuous amidase activity of an esterase from Bacillus subtilis, ChemBioChem, 9, 67-69, link.
[170] DiLorenzo, M., Hidalgo, A., Molina, R., Hermoso, J.A., Pirozzi, D., Bornscheuer, U.T. (2007), Enhancement of the stability of a prolipase from Rhizopus oryzae towards aldehydes by saturation mutagenesis, Appl. Environm. Microbiol. 73, 7291-7299, link.
[169] Hummel, A., Brüsehaber, E., Böttcher, D., Doderer, K., Trauthwein, H., Bornscheuer, U.T. (2007), Isoenzymes of pig liver esterase reveal striking differences in enantioselectivities, Angew. Chem. Int. Ed. 46, 8492-8494, link; Hummel, A., Brüsehaber, E., Böttcher, D., Doderer, K., Trauthwein, H., Bornscheuer, U.T. (2007), Isoenzyme der Schweineleberesterase zeigen bemerkenswerte Unterschiede in ihrer Enantioselektivität, Angew. Chem.119, 8644-8646, link.
[168] Gruber, C.C., Nestl, B.M., Gross., J., Hildebrandt, P., Bornscheuer, U.T., Faber, K., Kroutil, W. (2007), Emulation of racemase activity by employing a pair of stereocomplementary biocatalysts, Chem. Eur. J., 13, 8271-8276, link.
[167] Kourist, R., Krishna, S.H., Patel, J.S., Bartnek, F., Weiner, D.W., Hitchman, T., Bornscheuer, U.T. (2007), Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols, Org. Biomol. Chem., 5, 3310-3313, link.
[166] Brüsehaber, E., Böttcher, D., Musidlowska-Persson, A., Albrecht, D., Hecker, M., Doderer, K., Bornscheuer, U.T. (2007), Identification of pig liver esterase variants by tandem mass spectroscopy analysis and their characterization, Appl. Microbiol. Biotechnol., 76, 853-859, link.
[165] Rehdorf, J., Kirschner, A., Bornscheuer, U.T. (2007), Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440, Biotechnol. Lett., 29, 1393-1398, link.
[164] Kourist, R., Bartsch, S., Bornscheuer, U.T., (2007), Highly enantioselective synthesis of arylaliphatic tertiary alcohols using mutants of an esterase from Bacillus subtilis, Adv. Synth. Catal., 349, 1393-1398, link.
[163] Durban, M., Bornscheuer, U.T. (2007), An improved assay for the determination of phospholipase C activity, Eur. J. Lipid Sci. Technol., 109, 469-473, link.
[162] Geitner, K., Kirschner, A., Rehdorf, J., Schmidt, M., Mihovilovic, M.D., Bornscheuer, U.T. (2007), Enantioselective kinetic resolution of 3-phenyl-2-ketones using Baeyer-Villiger monooxygenases, Tetrahedron: Asymmetry, 18, 892-895, link.
[161] Kirschner, A., Altenbuchner, J., Bornscheuer, U.T. (2007), Design of a secondary alcohol degradation pathway from Pseudomonas fluorescens DSM50106 in an engineered E. coli, Appl. Microbiol. Biotechnol., 75, 1095-1101, link.
[160] Heinze, B., Kourist, R., Fransson, L., Hult, K., Bornscheuer, U.T. (2007), Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis, Protein Eng. Des. Sel., 20, 125-131, link.
[159] Bertram, M., C. Manschot-Lawrence, E. Flöter, Bornscheuer, U.T. (2007), A microtiter plate-based assay method to determine fat quality, Eur. J. Lipid Sci. Technol., 109, 180-185, link.
[158] Fotakopoulou, I., Barbayianni, E., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2007), Enzymatic removal of carboxyl protecting groups. Part III: Fast removal of the allyl and chloroethyl esters by Bacillus subtilis esterase (BS2), J. Org. Chem., 72, 782-786, link.
[157] Durban, M., Silbersack, J., Schweder, T., Schauer, F., Bornscheuer, U.T. (2007), High level expression of a recombinant phospholipase C from Bacillus cereus in B. subtilis, Appl. Microbiol. Biotechnol., 74, 634-639, link.
[156] Schmidt, M., Henke, E., Heinze, B., Kourist, R., Hidalgo, A., Bornscheuer, U.T. (2007), A versatile esterase from Bacillus subtilis: Cloning, expression, characterization, and its application in biocatalysis, Biotech. J., 2, 249-253, link.
[155] Böttcher, D., Brüsehaber, E., Doderer. K., Bornscheuer, U.T. (2007), Functional expression of the gamma-isoenzyme of pig liver carboxyl esterase in Escherichia coli, Appl. Microbiol. Biotechnol., 73, 1282-1289, link.
[154] Kirschner, A., Altenbuchner, J., Bornscheuer, U.T. (2007), Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas fluorescens DSM50106 in E.coli, Appl. Microbiol. Biotechnol., 73, 1065-1072, link.
[153] Böttcher, D., Bornscheuer, U.T. (2006), High-throughput screening of activity and enantioselectivity of esterases, Nature Protocols, 1, 2340-2343, online, link.
[152] Kirschner, A., Bornscheuer, U.T. (2006), Katalytische kinetische Racematspaltung von 4-Hydroxy-2-Ketonen durch eine Baeyer-Villiger Monooxygenase, Angew. Chem., 118, 7161-7163 (german version), link; Kirschner, A., Bornscheuer, U.T. (2006), Kinetic resolution of 4-hydroxy-2-ketones by a Baeyer-Villiger monooxygenase, Angew. Chem. Int. Ed.45, 7004-7006 (english version), link.
[151] Bellur, E., Freifeld, I., Holtz, E., Böttcher, D., Bornscheuer, U.T., Langer, P. (2006), Synthesis of (tetrahydrofuran-2-yl)acetates and (pyrrolidin-2-yl)acetates based on a 'cyclization / hydrogenation' strategy, Tetrahedron, 62, 7132-7139, link.
[150] Metzger, J.O., Bornscheuer, U.T. (2006), Lipids as renewable resources: Current state of chemical and biotechnological conversion and diversification, Appl. Microbiol. Biotechnol., 71, 13-22, link.
[149] Chaparro-Riggers, J.F., Breves, R., Maurer, K.H., Bornscheuer U.T. (2006), Modulation of infectivity in phage display as a tool to determine the substrate specificity of proteases, ChemBioChem., 7, 965-970, link.
[148] Bellur, E., Böttcher, D., Bornscheuer, U.T., Langer, P. (2006), Enantioselective synthesis of 2-alkylidenetetrahydrofurans based on a 'cyclization / enzymatic resolution' strategy, Tetrahedron: Asymmetry, 17, 892-899, link.
[147] Brandt, B., Hidalgo, A., Bornscheuer, U.T. (2006), Immobilization of enzymes in the microtiter plate scale, Biotech. J., 1, 582-587, link.
[146] Schmidt, M., Hasenpusch, D., Kähler, M., Kirchner, U., Wiggenhorn, K., Langel, W., Bornscheuer, U.T. (2006), Directed evolution of an esterase from Pseudomonas fluorescens yields a mutant with excellent enantioselectivity and activity for the kinetic resolution of a chiral building block, ChemBioChem., 7, 805-809, link.
[145] Ganske, F., Bornscheuer, U.T. (2006), Growth of Escherichia coli, Pichia pastoris and Bacillus cereus in the presence of the ionic liquids [BMIM][BF4] and [BMIM][PF6] and organic solvents, Biotechnol. Lett., 28, 465-469, link.
2001 - 2005
[144] Xu, J.-H., Zhou, R., Bornscheuer, U.T. (2005), Comparison of differently modified Pseudomonas cepacia lipases in enantioselective preparation of a chiral alcohol for agrochemical use, Biocat. Biotransform., 23, 415-422, link.
[143] Di Lorenzo, M., Hidalgo, A., Haas, M., Bornscheuer, U.T. (2005), Heterologous production of functional Rhizopus oryzae lipase forms in Eschericha coli, Appl. Environm. Microbiol., 71, 8974-8977, link.
[142] Sareen, R., Bornscheuer, U.T., Mishra, P., Cloning, functional expression and characterization of an alkaline, organic solvent tolerant protease from Bacillus licheniformis RSP-09-37, Biotechnol. Lett., 27, 1901-1907, link.
[141] Poessl, T.M., Kosjek, B., Ellmer, U., Gruber, C.C., Edegger, K., Faber, K. Hildebrandt, P., Bornscheuer, U.T., Kroutil, W. (2005), Stereo-complementary non-racemic halohydrins via biocatalytic hydrogen transfer-reduction of halo-ketones and one-pot cascade-reaction to enantiopure epoxide, Adv. Synth. Catal., 345, 1827-1834, link.
[140] Ödman, P., Wessjohann, L.A., Bornscheuer, U.T. (2005), Chemoenzymatic dynamic kinetic resolution of acyloins, J. Org. Chem., 70, 9551-9555, link.
[139] Barbayianni, E., Fotakopoulou, I., Schmidt, M., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2005), Enzymatic removal of carboxyl protecting groups. Cleavage of the benzyl- and methyl moieties, J. Org. Chem., 70, 8730-8733, link.
[138] Ganske, F., Bornscheuer, U.T. (2005), Optimization of lipase-catalyzed glucose fatty acid ester synthesis in a two-phase systems containing ionic liquids and t-BuOH, J. Mol. Catal. B: Enzym., 36, 40-42, link.
[137] Bornscheuer, U.T., Rodriguez Ordonez, G., Hidalgo, A., Gollin, A., Lyon, J., Hitchman, T.S., Weiner, D.P. (2005), Selectivity of lipases and esterases towards phenol esters, J. Mol. Catal. B: Enzymatic, 36, 8-13, link.
[136] Buchholz, K., Bornscheuer, U.T. (2005), Highlights in biocatalysis – Historical landmarks and current trends, Eng. Life Sci., 5, 309-323, link.
[135] Bornscheuer, U.T. (2005), Deep-sea mining for unique biocatalysts, Chem. Biol., 12, 859-860, link.
[134] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2005), Synthesis of ascorbyloleate by immobilized Candida antarctica lipases, Process Biochemistry, 40, 3177-3180, link.
[133] Bornscheuer U.T. (2005), White biotechnology for lipids, fats and oils, Eur. J. Lipid Sci. Technol., 107, 445-446, link.
[132] Chaparro-Riggers, J.F., Breves, R., Michels, A., Maurer, K.H., Bornscheuer U.T. (2005), A GFP-based assay for the determination of hydrolytic activity and substrate specificity of subtilisins under washing conditions, J. Mol. Catal. B: Enzymatic, 35, 74-77, link.
[131] Ganske, F., Bornscheuer, U.T. (2005), Lipase-catalyzed glucose fatty acid ester synthesis in ionic liquids, Org. Lett., 7, 3097-3098, link.
[130] Strübing, D., Kirschner, A., Neumann, H., Hübner, S., Klaus, S., Bornscheuer, U.T., Beller, M. (2005), Synthesis of enantiomerically pure cyclohex-2-en-1-ols: Development of novel multicomponent reactions, Chem. Eur. J., 11, 4210-4218, link.
[129] Schmidt, M., Bornscheuer, U.T. (2005), High-throughput assays for lipases and esterases, Biomolecular Eng., 22, 51-56, link.
[128] Robertson, D.E., Bornscheuer, U.T. (2005), Biocatalysis and biotransformation. New technologies, enzymes and challenges, Curr. Opin. Chem. Biol., 9, 164-165, link.
[127] Schmidt, M., Barbayianni, E., Fotakopoulou, I., Höhne, M., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2005), Enzymatic Removal of Carboxyl Protecting Groups. Part I: Cleavage of the tert-Butyl Moiety, J. Org. Chem., 70, 3737-3040, available online as ASAP article, link.
[126] Soumanou, M., Aleodjrodo, P., Bornscheuer, U.T. (2004), Activity and stability of immobilized lipases in lipase-catalyzed modification of peanut oil, Oleagineux, Corps Gras, Lipides, 11, 464-468.
[125] Sareen, R., Bornscheuer, U.T., Mishra, P. (2004), Synthesis of Kyotorphin precursor by an organic solvent–stable protease from Bacillus licheniformis RSP-09-37, J. Mol. Catal. B: Enzym., 32, 1-5, link.
[124] Bornscheuer, U.T. (2004), High-throughput-screening systems for hydrolases, Eng. Life. Sci.,4, 539-542, link.
[123] Bornscheuer, U.T., Kazlauskas, R.J. (2004), Catalytic promiscuity in biocatalysis: Using old enzymes to form new bonds and follow new pathways, Angew. Chem. Int. Ed. Engl., 42, 6032-6040, link; Untreue Enzyme in der Biokatalyse: Mit alten Enzymen zu neuen Bindungen und Synthesewegen, Angew. Chem., 116, 6156-6165, link.
[122] Wongsakul, S., Bornscheuer, U.T. Kittikun, H. (2004), Lipase-catalyzed acidolysis and Phospholipase D-catalyzed transphosphatidylation of phosphatidylcholine, Eur. J. Lipid Sci. Technol., 106, 665-670, link.
[121] Scheid, G., Ruijter, E., Konarzycka-Bessler, M., Bornscheuer, U.T., Wessjohann, L.A. (2004), Synthesis and resolution of a key building block for epothilones – a comparison of asymmetric synthesis, chemical, and enzymatic resolution, Tetrahedron: Asymmetry, 15, 2861-2869, link.
[120] Kirschner, A., Langer, P., Bornscheuer, U.T. (2004), Lipase-catalyzed highly enantioselective kinetic resolution of alpha-hydroxy butenolides, Tetrahedron: Asymmetry, 15, 2871-2874, link.
[119] Bornscheuer, U.T. (2004), Finding enzymatic gold on silver surfaces, Nat. Biotechnol., 22, 1098-1099, link.
[118] Sareen, R., Bornscheuer, U.T., Mishra, P. (2004), A microtiter plate assay for the determination of the synthetic activity of protease, Anal. Biochem., 333, 193-195, link.
[117] Schmidt, M., Baumann, M., Henke, E., Konarzycka-Bessler, M., Bornscheuer U.T. (2004), Directed evolution of lipases and esterases, Methods Enzymol., 388, 199-207.
[116] Wongsakul, S., Kittikun, A., Bornscheuer, U.T. (2004), Lipase-catalyzed synthesis of structured triglycerides from 1,3-diglycerides, J. Am. Oil Chem. Soc., 81, 151-155.
[115] Schönfeld, D.L., Bornscheuer, U.T. (2004), A polarimetric assay for the medium-throughput determination of amino acid racemase activity, Anal. Chem., 76, 1184-1188, link.
[114] Wessjohann, L.A., Scheid, G., Orru, R.V.A., Kuit, W., Ruijter, E., Henke, E., Bornscheuer, U.T. (2004), A new route to protected acyloins and their enzymatic resolution with lipases, Eur. J. Org. Chem., 1063-1074, link.
[113] Musidlowska-Persson, A., Bornscheuer, U.T. (2003), Recombinant porcine intestinal carboxyl esterase: Cloning from the pig liver esterase gene by site-directed mutagenesis, functional expression and characterization, Protein Eng., 16, 1139-1145, link.
[112] Soumanou, M.M., Bornscheuer, U.T. (2003), Lipase-catalyzed alcoholysis of vegetable oils, Eur. J. Lipid Sci. Technol.,105, 656-660, link.
[111] Durban, M., Bornscheuer, U.T. (2003), An assay system for the detection of Phospholipase C activity, Eur. J. Lipid Sci. Technol., 105, 633-637, link.
[110] Ganske, F., Meyer, H.H., Deutz, H., Bornscheuer, U.T. (2003), Enzyme-catalyzed hydrolysis of 18-methyl eicosanoic acid-cysteine thioester, Eur. J. Lipid Sci. Technol., 105, 627-632, link.
[109] Bornscheuer, U.T., Musidlowska-Persson, A., Böttcher, D., Liebeton, K., Lorenz, P., Eck, J., Zinke, H., Schleper, C., Langer, P., Trauthwein, H., Karau, A., Buchholz, S. (2003), Esterasen und Lipasen aus kultivierten und nicht-kultivierten Mikroorganismen, Transkript, 9 (10), 81-84.
[108] Kähler, M., Rieks, A., Kirchner, U., Wiggenhorn, K., Bornscheuer, U.T., Schmidt, M., Eisner, A., Petersen, W., Ellerbrock, F., Bollow, S. (2003), Biokatalytische Synthese enantiomerenreiner building blocks, Transkript, 9 (10), 78-80.
[107] Bornscheuer, U.T. (2003), Immobilizing enzymes: how to create more suitable biocatalysts, Angew. Chem. Int. Ed. Engl.42, 3336-3337, link; Enzymimmobilisierung: ein Weg zu verbesserten Biokatalysatoren, Angew. Chem., 115, 3458-3459, link.
[106] Soumanou, M.M., Bornscheuer, U.T. (2003), Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil to Biodiesel using immobilized lipases, Enzyme Microb. Technol., 33, 97-103, link.
[105] Henke, E., Bornscheuer, U.T., Schmid, R.D., Pleiss, J. (2003), The molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases, Chem. Bio. Chem., 4, 485-493, link.
[104] Musidlowska-Persson, A., Bornscheuer, U.T. (2003), Site-directed mutagenesis of recombinant pig liver esterase
yields mutants with altered enantioselectivity, Tetrahedron: Asymmetry, 14, 1341-1344, link.
[103] Horsman, G.P., Liu, A.F.M., Henke, E., Bornscheuer, U.T., Kazlauskas, R.J. (2003), Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase toward methyl 3-Bromo-2-methylpropanoate and ethyl 3-phenylbutyrate, Chem. Eur. J., 9, 1933-1939, link.
[102] Konarzycka-Bessler, M. Bornscheuer, U.T. (2003), A high-throughput-screening method for the determination of the synthetic activity of hydrolases, Angew. Chem. Int. Ed., 42, 1418-1420, link; Eine Hochdurchsatz-Screeningmethode zur Bestimmung der Syntheseaktivität von Hydrolasen, Angew. Chem., 115, 1449-1451, link.
[101] Persson, M.M., Bornscheuer, U.T. (2003), Increased stability of an esterase from Bacillus subtilis in ionic liquids as compared to organic solvents, J. Mol. Catal. B: Enzymatic, 22, 21-27, link.
[100] Wongsakul, S., Prasertsan, P., Bornscheuer, U.T., H-Kittikun, A. (2003), Synthesis of 2-monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases, Eur. J. Lipid Sci. Technol., 105, 68–73, link.
[99] Henke, E., Bornscheuer, U.T. (2003), Fluorophoric assay for the high-throughput determination of amidase activity, Anal. Chem., 75, 255-260 , link.
[98] Hari Krishna, S., Persson, M.M., Bornscheuer, U.T. (2002), Enantioselective transesterification of a tertiary alcohol by Lipase A from Candida antarctica, Tetrahedron: Asymmetry, 13, 2693-2696.
[97] Bornscheuer, U.T. (2002), Methods to increase enantioselectivity of lipases and esterases, Curr. Opin. Biotechnol., 13, 543-547.
[96] Vorlová, Bornscheuer, U.T., Gatfield, I., Hilmer, J.M., Bertram, H.J., Schmid, R.D., (2002), Enantioselective hydrolysis of D,L-menthyl benzoate to L-menthol by recombinant Candida rugosa lipases, Adv. Synth. Catal., 344, 1152-1155, link.
[95] Henke, E., Bornscheuer, U.T., (2002), Esterases from Bacillus subtilis and Bacillus stearothermophilus share high sequence homology but differ substantially in their properties, Appl. Microbiol. Biotechnol., 60, 320-326, link.
[94] Musidlowska-Persson, A., Bornscheuer, U.T. (2002), Substrate specificity of the gamma-isoenzyme of recombinant pig liver esterase towards acetates of secondary alcohols, J. Mol. Catal. B: Enzymatic, 19-20C, 129-133.
[93] Bornscheuer, U.T., Bessler, C., Srinivas, R., Krishna, S.H. (2002), Optimizing lipases and related enzymes for efficient application, Trends Biotechnol., 20, 433-437.
[92] Henke, E., Pleiss, J., Bornscheuer, U.T. (2002), Activity of lipases and esterases towards tertiary alcohols: Insights into structure-function relationships, Angew. Chem. Int. Ed., 41, 3211-3213. Aktivität von Lipasen und Esterasen gegenüber tertiären Alkoholen: Neue Einblicke in Struktur-Funktions-Beziehungen, Angew. Chem., 114, 3338-3341, ,supporting information: PDF.
[91] Hildebrandt, P., Musidlowska-Persson, A., Bornscheuer, U.T., Altenbuchner, J. (2002), Cloning, functional expression and biochemical characterization of a stereoselective dehydrogenase from Pseudomonas fluorescens DSM50106, Appl. Microbiol. Biotechnol., 59, 483-487.
[90] Bornscheuer, U.T. (2002), Microbial carboxyl esterases - Classification, properties and application in biocatalysis, FEMS Microbiol. Rev., 26, 73-81.
[89] Henke, E., Baumann, M., Musidlowska-Persson, A., Bornscheuer, U.T. (2002), Innovative Methoden zur Erzeugung stereoselektiver Esterasen, BIOforum, 4, 218-219.
[88] Yan, Y., Bornscheuer, U.T., Schmid, R.D. (2002), Efficient water removal in lipase-catalyzed esterifications using a low-boiling-point azeotrope, Biotechnol. Bioeng., 78, 31-34, link.
[87] Baumann, M., Stürmer, R., Bornscheuer, U.T. (2001), A high-throughput-screening method for the identification of active and enantioselective hydrolases, Angew. Chem. Int. Ed., 40, 4201-4204, link. Eine Hochdurchsatz-Screening-Methode zur Identifizierung aktiver und enantioselektiver Hydrolasen, Angew. Chem., 113, 4329-4333, link, HOT PAPER.
[86] Bornscheuer, U.T. (2001), Lipase-catalyzed synthesis of structured triglycerides, Lipid Technology, 7, 105-107.
[85] Yan, Y., Stadler, G., Lutz-Wahl, S., Otto, R.T., Reuss, M., Bornscheuer, U.T., Schmid, R.D. (2001), Regioselective glucose ester synthesis on preparative scale, Eur. J. Lipid Sci. Technol., 103, 583-587, link.
[84] Lange, S., Musidlowska,A., Schmidt-Dannert, C., Schmitt, J., Bornscheuer, U.T. (2001), Cloning,functional expression and characterization of recombinant pig liver esterase, ChemBioChem.,2, 576-582, link
[83] Musidlowska, A., Lange, S., Bornscheuer, U.T. (2001), Via Overexpression in the yeast Pichia pastoris to enhanced enantioselectivity: New aspects in the application of pig liver esterases, Angew. Chem. Int. Ed., 40, 2851-2853, link. Durch Überexpression in der Hefe Pichia pastoris zu erhöhter Enantioselektivität: Neue Aspekte in der Anwendung von Schweineleberesterase, Angew. Chem., 113, 2934-2936, link.
[82] Hildebrandt, P., Riermeier, T., Altenbuchner, J., Bornscheuer, U.T. (2001), Efficient resolution of prostereogenic arylaliphatic ketones using a recombinant alcohol dehydrogenase from Pseudomonas fluorescens DSM50106 expressed in E. coli, Tetrahedron: Asymmetry, 12, 1207-1210.
[81] Bornscheuer, U.T. (2001), Directed evolution of enzymes for biocatalytic applications, Biocatal. Biotransform., 19, 84-96.
[80] Liu, A. M. F., Somers, N. A., Kazlauskas, R. J., Brush, T. S., Zocher, F., Enzelberger, M. M., Bornscheuer, U. T., Horsman, G. P., Mezzetti, A., Schmidt-Dannert, C., Schmid, R. D. (2001), Mapping the substrate selectivity of new hydrolases using colorimetric screening:lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from Pseudomonas fluorescens and Streptomyces diastatochromogenes, Tetrahedron: Asymmetry, 12, 545-556.
[79] Bornscheuer,U. T.,Pohl, M. (2001), Improved biocatalysts by directed evolution and rationalprotein design, Curr. Opin. Chem. Biol.5, 137-142. With permission from Elsevier Science, single copies of the article may be downloaded and printed for the reader's personal research and study here.
[78] Yan, Y., Bornscheuer, U. T., Stadler, G., Lutz-Wahl, S., Reuss, M., Schmid, R. D. (2001), Production of sugar fatty acid esters by enzymatic esterification in a stirred-tankmembrane reactor: Optimization of parameters by response surface methodology, J. Am. Oil Chem. Soc.78, 147-152.
   
1996 - 2000
[77] Yan, Y., Bornscheuer, U.T., Stadler, G., Reuss, R., Schmid, R.D. (2000), Enzymatic productionof biosurfactant 6-O-glucose fatty acid monoester, J. Deterg. Cosmetics (in Chinese) 23, 71-77.
[76] Baumann, M., Hauer,B.H., Bornscheuer, U.T. (2000), Rapid screening of hydrolases for the enantioselective conversion of "difficult-to-resolve substrates", Tetrahedron: Asymmetry11, 4781-4790, supporting information (PDF).
[75] Bornscheuer, U. T. (2000), Directed evolution of enzymes, Chim. Oggi / Chem. Today, 18(9), 65-67.
[74] Stander, M. A., Bornscheuer, U. T., Henke, E., Steyn, P. S. (2000), Screening of commercial hydrolases for the degradation of Ochratoxin A, J. Agric. Food Chem.48, 5736-5739, link.
[73] Peters, H., Schmidt-Dannert, C., Cao, L., Bornscheuer, U.T., Schmid, R.D. (2000), Purification and reconstitution of an integral membrane protein, the photoreaction center of Rhodobacter sphaeroides, using synthetic sugar esters, Biotechniques28, 1214-1219.
[72] Henke, E., Schuster, S., Yang, H., Bornscheuer, U.T., (2000), Lipase-catalyzed resolution of Ibuprofen, Chem. Month.131, 633-638.
[71] Otto, R., Scheib, H., Bornscheuer, U.T., Pleiss, J., Syldatk, C., Schmid, R.D. (2000), Substrate specificity of lipase B from Candida antarctica in the synthesis of arlyaliphatic glycolipids, J. Mol. Catal. B: Enzymatic8,210-211.
[70] Yan, Y., Bornscheuer, U.T., Schmid, R.D. (1999), Lipase-catalyzed synthesis of vitamin C fattyacid esters, Biotechnol. Lett.21, 1051-1054.
[69] Zocher, F., Enzelberger, M.M., Bornscheuer, U.T., Hauer, B., Wohlleben, W., Schmid, R.D. (1999),Epoxide hydrolase activity of Streptomyces strains, J. Biotechnol.77 ,287-292.
[68] Wolfangel, P., Meyer, H.H., Bornscheuer, U.T., Müller, K. (1999), Solid state NMR and FTIR studies on bilayer membranes from 1,2-dioctadec-(14-ynoyl)-sn-glycero-3-phosphatidylcholine, Biochem.Biophys. Acta1420, 121-138.
[67] Henke, E., Bornscheuer, U.T. (1999), Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay, Biol. Chem.380, 1029-1033.
[66] Yan, Y., Bornscheuer, U.T., Cao, L., Schmid, R.D. (1999), Lipase-catalyzed solid phase synthesis of sugar fatty acid esters. Removal of by-products by azeotropic distillation, Enzyme Microb. Technol.25, 725-728.
[65] Bornscheuer, U.T., Altenbuchner, J., Meyer., H.H. (1999), Directed evolution of an esterase: Screening of enzyme libraries based on pH-indicators and a growth assay, Bioorg.Med. Chem.7, 2169-2173.
[64] Schmid, U., Bornscheuer, U.T., Soumanou, M.M., McNeill, G.P., Schmid, R.D. (1999), Highly-selective synthesis of 1,3-oleyl-2-palmitoyl-glycerol by lipase catalysis, Biotechnol. Bioeng.64, 678-684.
[63] Ji, H.-S., McNiven,S., Yano, K., Ikebukuro, K., Bornscheuer, U.T., Schmid, R.D., Karube, I.(1999), Highly sensitive trilayer piezoelectric odor sensor, Anal. Chim.Acta387, 39-45.
[62] Zocher, F., Enzelberger, M.M., Bornscheuer, U.T., Hauer, B., Schmid, R.D. (1999), A colorimetric assay suitable for screening epoxide hydrolase activity, Anal. Chim. Acta391, 345-351.
[61] Khalameyzer, V., Bornscheuer, U.T. (1999), Overexpression and characterization of an esterase from Streptomyces diastatochromogenes, Biotechnol. Lett.21, 101-104.
[60] Yang, H., Henke, E., Bornscheuer, U.T. (1999), Highly efficient double enantioselection by lipase-catalyzed transesterification of (R,S)-carboxylic acid vinyl esters with ( R,S)-1-phenylethanol,Tetrahedron:Asymmetry10, 957-960.
[59] Yang, H., Henke, E., Bornscheuer, U.T. (1999), The use of vinyl esters significantly enhancedenantioselectivities and reaction rates in lipase-catalyzed resolutions of arlyaliphatic carboxylic acids, J. Org. Chem.64, 1709-1712.
[58] Cao, L., Bornscheuer, U.T., Schmid, R.D. (1999), Lipase-catalyzed solid-phase synthesis of sugar esters. Influence of immobilization on productivity and stability of the enzyme, J. Mol. Catal. B: Enzymatic6, 279-285.
[57] Khalameyzer, V., Fischer, I., Bornscheuer, U.T., Altenbuchner, J. (1999), Screening, nucleotide sequence and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity toward lactones, Appl. Environm. Microbiol.65,477-482.
[56] Minning, S., Weiss,A., Bornscheuer, U.T., Schmid, R.D. (1999), Determination of peracid and putative enzymatic peracid formation by an easy colorimetric assay, Anal.Chim.Acta378, 295-300.
[55] Soumanou, M.M., Bornscheuer, U.T., Schmid, U., Schmid, R.D. (1999), Crucial role of support and water activity on the lipase-catalyzed synthesis of structured triglycerides, Biocatal. Biotransform.16, 443-459.
[54] Cao, L., Bornscheuer, U.T., Schmid, R.D. (1998), Lipase-catalyzed solid phase synthesis of sugar esters, IV: Selectivity of lipases towards primary and secondary hydroxyl groups in carbohydrates, Biocatal. Biotransform.16, 249-257.
[53] Bornscheuer, U.T. (1998), Directed evolution of enzymes, Angew. Chem. / Angew. Chem. Int. Ed. Engl. 110 / 37, 3285-3288 / 3105-3108.
[52] Schmid, U., Bornscheuer, U.T., Soumanou, M.M., McNeill, G.P., Schmid, R.D. (1998), Optimization of the reaction conditions in the lipase-catalyzed synthesis of structured triglycerides, J. Am. Oil Chem. Soc.75, 1527-1531.
[51] Otto, R.T., Bornscheuer, U.T., Scheib, H., Pleiss, J., Syldatk, C., Schmid, R.D. (1998), Lipasecatalyzed esterification of unusual substrates: Synthesis of glucuronic acid and ascorbic acid (vitamin C) esters, Biotechnol. Lett.20,1091-1094.
[50] Takahashi, S., Ueda, M., Atomi, H., Beer, H.D., Bornscheuer, U.T., Schmid, R.D., Tanaka, A. (1998), Extracellular production of active Rhizopus oryzae lipase by Saccharomyces cerevisiae, J. Ferment. Bioeng.86, 164-168.
[49] Otto, R., Bornscheuer, U.T., Syldatk, C., Schmid, R.D. (1998), Lipase-catalyzed synthesis of arylaliphatic esters of b-D(+)-glucose, alkyl- and arylglucosides and characterizationof their surfactant properties, J. Biotechnol.64, 231-237.
[48] Beer, H.D., Bornscheuer, U.T., McCarthy, J.E.G., Schmid, R.D. (1998), Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae, Biochim.Biophys. Acta1399, 173-180.
[47] Bornscheuer, U., Heinz, E. (1998), Toward new fats, oils through biotechnology, Inform9, 485-488.
[46] Wagegg, T., Enzelberger, M.M., Bornscheuer, U.T., Schmid, R.D. (1998), The use of methoxy acetoxyesters significantly enhances reaction rates in the lipase-catalyzed preparation of optical pure 1-(4-chloro-phenyl) ethyl amines, J. Biotechnol.61,75-78.
[45] Enzelberger, M.M., Zocher, F., Schmidt-Dannert, C., Bornscheuer, U.T., Schmid, R.D., Hauer, B., Eipel, H. (1998), Singularization of enzyme mutants by use of a cell sorter andgreen fluorescent protein, BIOforum4, 192-194.
[44] Otto, R.T., Bornscheuer, U.T., Syldatk, C., Schmid, R.D. (1998), Synthesis of aromatic n-alkyl-glucoside esters in a coupled ß-glucosidase and lipase reaction, Biotechnol. Lett.20, 437-440.
[43] Soumanou, M.M., Bornscheuer, U.T., Schmid, R.D. (1998), Two-step enzymatic reaction for the synthesisof pure structured triglycerides, J. Am. Oil Chem. Soc.75,703-710.
[42] Soumanou, M.M., Bornscheuer, U.T., Schmid, U., Schmid, R.D. (1998), Synthesis of structured triglycerides by lipase catalysis, Fett/Lipid100, 156-160.
[41] Krebsfänger, N., Zocher, F., Altenbuchner, J., Bornscheuer, U.T. (1998), Characterization, and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens, Enzyme Microb. Technol.22, 641-646.
[40] Yano, K., Bornscheuer, U.T., Schmid, R.D., Yoshitake, H., Ikebukuro, K., Yokoyama, K., Masuda,Y., Karube, I. (1998), Development of odorant sensor using polymer-coatedquartz crystals immobilized with unusual lipids, Biosensors Bioelectronics13,397-405.
[39] Zocher, F., Krebsfänger, N., Yoo, O.J., Bornscheuer, U.T. (1998), Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens, J. Mol. Catal.B, Enzymatic5, 187-190.
[38] Bornscheuer, U.T., Altenbuchner, J., Meyer, H.H. (1998), Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of Epothilones, Biotechnol. Bioeng.58, 554-559, link.
[37] Krebsfänger, N., Schierholz, K., Bornscheuer, U.T. (1998), Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens towards alcohols and carboxylic acids, J. Biotechnol.60, 105-111.
[36] Bornscheuer, U.T., Enzelberger, M.M., Altenbuchner, J., Meyer, H.H. (1998), Mutator strain Epicuriancoli XL1-red generates esterase variants, Strategies11, 16-17.
[35] Kisselev, P., Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D., Schwarz, D. (1998), Branched phosphatidylcholines stimulate activity of cytochrome P450SCC (CYP11A1)in phospholipid vesicles by enhancing cholesterol binding, membrane incorporation, and protein exchange, J. Biol. Chem.273, 1380-1286.
[34] Yano, K., Yoshitake, H., Bornscheuer, U.T., Schmid, R.D., Ikebukuro, K., Yokoyama, K., Masuda, Y., Karube, I. (1997), Development of a chemical vapor sensor using piezoelectric quartz crystals coated with unusual lipids, Anal. Chim. Acta340 ,41-48.
[33] Thude, S., Shukun, L., Said, M.B., Bornscheuer, U.T. (1997), Lipase-catalyzed synthesis of monoacylglycerides by glycerolysis of campher tree seed oil and cocoa-butter, Fett/Lipid99,246-250.
[32] Blüher, A., Grube, A., Bornscheuer, U., Banik, G. (1997), A reappraisal of the enzyme lipase for removing drying-oil stains on paper, Paper Conservator21 ,37-47.
[31] Soumanou, M.M., Bornscheuer, U.T., Menge, U., Schmid, R.D. (1997), Synthesis of structured triglycerides from peanut oil with immobilized lipase, J. Am. Oil Chem. Soc.74,427-433.
[30] Cao, L., Fischer, A., Bornscheuer, U.T., Schmid, R.D. (1997), Lipase-catalyzed solid phase preparation of sugar fatty acid esters, Biocatal. Biotransform.14, 269-283.
[29] Schwarz, D., Kisselev, P., Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Possible involvement of nonbilayer lipids in the stimulation of the activity ofcytochrome P450SCC (CYP11A1) and its propensity to induce vesicle aggregation,Chem. Phys. Lipids85, 91-99.
[28] Pisch, S., Bornscheuer, U., Meyer, H.H., Schmid, R.D. (1997), Properties of unusual phospholipids IV: Chemoenzymatic synthesis of phospholipids bearing acetylenic fatty acids, Tetrahedron53, 14627-14634.
[27] Negelmann, L., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Properties of unusual phospholipids III: Synthesis, monolayer investigations and DSC studies of hydroxy octadeca(e)noic acids and diacylglycerophosphocholines derived therefrom, Chem. Phys. Lipids90, 117-134.
[26] Schwarz, D., Kisselev, P., Pfeil, W., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Evidencethat nonbilayer phase propensity of the membrane is important for the sidechain cleavage activity of cytochrome P450SCC (CYP11A1), Biochemistry36,14262-14270.
[25] Enzelberger, M.M., Bornscheuer, U.T., Gatfield, I., Schmid, R.D. (1997), Lipase-catalysed resolution of g-and d-lactones, J. Biotechnol.56, 129-133.
[24] Gaziola, L., Bornscheuer, U., Schmid, R.D. (1996), A rapid and effective separation of enantiomers of glycerol derivatives by gas chromatography and their lipase-catalyzed biotransformation, Enantiomer1, 49-54.
[23] Wünsche, K., Schwaneberg, U., Bornscheuer, U.T., Meyer, H.H. (1996), Chemoenzymic route to b-blockers via 3-hydroxy esters, Tetrahedron: Asymmetry7, 2017-2022.
[22] Bornscheuer, U., Gaziola, L., Schmid, R.D. (1996), Application of vinyl esters for the lipase-catalyzed high-yield synthesis of monoacylglycerols, Ann. N.Y. Acad. Sci.799,757-761.
[21] Lampe, T.F.J., Hoffmann, H.M.R., Bornscheuer, U.T. (1996), Lipase mediated desymmetrization of meso 2,6- di(acetoxymethyl)tetrahydropyran-4-one derivatives. An innovative route to enantiopure 2,4,6-trifunctionalized C-glycosides, Tetrahedron:Asymmetry7, 2889-2900.
[20] Bornscheuer, U., Capewell, A., Wendel, V., Scheper, T. (1996), Online determination of the conversion in a lipase-catalyzed kinetic resolution in supercritical carbon dioxide, J. Biotechnol.46, 139-143.
[19] Heidt, M., Bornscheuer, U., Schmid, R.D. (1996), Studies on the enantioselectivity in the lipase-catalyzed synthesis of monoacylglycerols from the isopropylidene glycerol, Biotechnol. Tech.10, 25-30.
[18] Cao, L., Bornscheuer, U.T., Schmid, R.D. (1996), Lipase-catalyzed solid-phase synthesis of sugar esters, Fett/Lipid98, 332-335.
[17] Capewell, A., Wendel, V., Bornscheuer, U., Meyer, H.H., Scheper, T. (1996), Lipase-catalyzed kinetic resolution of 3-hydroxy esters in organic solvents and supercritical carbon dioxide, Enzyme Microb. Technol.19, 181-186.
1991 - 1995
[16] Bornscheuer, U., Herar, A., Capewell, A., Wendel, V., Kreye, L., Scheper, T., Voß, E., Wünsche, K., Meyer, H.H. (1995), Lipase-catalyzed kinetic resolution of 3-hydroxyesters: Optimization, batch, and continuous reactions, Ann. N.Y. Acad.Sci.750, 215-221.
[15] Bornscheuer, U.T., Yamane, T. (1995), Fatty acid vinyl esters as acylating agents: a new method for the enzymic synthesis of monoacylglycerols, J. Am. Oil Chem. Soc.72, 193-197.
[14] Bornscheuer, U.T. (1995), Lipase-catalyzed syntheses of monoacylglycerols, Enzyme Microb. Technol.17, 578-586.
[13] Bornscheuer, U. (1995), Lipase-catalyzed synthesis of monoglycerides, Fat Sci. Technol.97, 241-249.
[12] Ballesteros, A., Bornscheuer, U., Capewell, A., Combes, D., Condoret, J.-S., König, K., Kolisis, F.N., Marty, A., Menge, U., Scheper, T., Stamatis, H., Xenakis, A. (1995), Review article: Enzymes in non-conventional phases, Biocatal. Biotransform.13, 1-42.
[11] Heß, R., Bornscheuer, U., Capewell, A., Scheper, T. (1995), Lipase-catalyzed synthesis of monostearoylglycerol in organic solvents from 1,2-O-isopropylidene glycerol,Enzyme Microb. Technol.17, 725-728.
[10] Bornscheuer, U., Stamatis, H., Xenakis, A., Yamane, T., Kolisis, F.N. (1994), A comparison of different strategies for lipase-catalyzed synthesis of partial glycerides, Biotechnol. Lett.16, 697-702.
[9] Bornscheuer, U.T., Yamane, T. (1994), Activity and stability of lipase in the solid-phase glycerolysis of triolein, Enzyme Microb. Technol.16, 864-869.
[8] Bornscheuer, U., Reif, O.-W., Lausch, R., Freitag, R., Scheper, T., Kolisis, F.N., Menge, U. (1994), Lipase of Pseudomonas cepacia for biotechnological purposes: purification, crystallization and characterization, Biochim. Biophys. Acta1201, 55-60.
[7] Kreye, L., Herar, A., Bornscheuer, U., Scheper, T. (1994), Enzymic conversions in organic solvents in batch and continuous systems, Fat Sci. Technol.96, 246-251.
[6] Stamatis, H., Xenakis, A., Bornscheuer, U., Scheper, T., Menge, U., Kolisis, F.N. (1993), Pseudomonas cepacia lipase: esterification reactionsin AOT microemulsion systems,Biotechnol. Lett.15, 703-708.
[5] Bornscheuer, U., Herar, A., Kreye, L., Wendel, V., Capewell, A., Meyer, H.H., Scheper, T., Kolisis, F.N. (1993), Factors affecting the lipase catalyzed transesterification reactions of 3-hydroxy esters in organic solvents, Tetrahedron:Asymmetry4, 1007-1016.
[4] Capewell, A., Bornscheuer, U., Herar, A., Scheper, T., Meyer, H.H., Kolisis, F. (1992), A comparison of enzymic reactions in aqueous, organic and supercritical phases, DECHEMA Biotechnol. Conf.5, 57-60.
[3] Bornscheuer, U., Schapöhler, S., Scheper, T., Schügerl, K., König, W.A. (1992), Application of enantioselective capillary gas chromatography in lipase-catalyzed transesterification reactions in organic media, J. Chromatogr.606, 288-290.
[2] Bornscheuer, U., Capewell, A., Scheper, T., Meyer, H.H. (1992), A comparison of enzymic reactions in aqueous, organic, and supercritical phases, Ann. N.Y. Acad. Sci.672, 336-342.
[1] Bornscheuer, U., Schapöhler, S., Scheper, T., Schügerl, K. (1991), Influences of reaction conditions on the enantioselective transesterification using Pseudomonas cepacia lipase, Tetrahedron: Asymmetry2, 1011-1014.